The Rat Transferrin Receptor (TFR) ELISA Kit is a reliable tool for the precise measurement of TFR levels in rat samples such as serum, plasma, and tissue homogenates. This kit offers exceptional sensitivity and specificity, ensuring accurate and reproducible results for a variety of research applications.The transferrin receptor is essential for the regulation of iron homeostasis in the body, responsible for the uptake of transferrin-bound iron into cells. Dysregulation of TFR expression has been linked to various diseases, including iron deficiency anemia, neurodegenerative disorders, and cancer.
Therefore, studying TFR levels can provide valuable insights into these conditions and aid in the development of potential treatments.Overall, the Rat Transferrin Receptor (TFR) ELISA Kit is a valuable tool for researchers looking to investigate the role of TFR in health and disease, providing accurate and reliable data for advancing scientific knowledge and potential therapeutic strategies.
Product Name:
Rat Transferrin Receptor (TFR) ELISA Kit
Product Code:
RTDL01014
Size:
96 Assays
Target:
TFR
Synonyms:
CD71, TFR1, P90, TFRC, T9, Trfr, sTfR, Transferrin receptor protein 1, serum form
Detection Method:
Sandwich
Range:
0.625-40ng/mL
Shelf Life:
12 months
Note:
For research use only
Recovery:
Matrices listed below were spiked with certain level of recombinant the index and the recovery rates were calculated by comparing the measured value to the expected amount of the index in samples.
Matrix
Recovery range (%)
Average(%)
Serum (n=5)
80-102
91
EDTA plasma (n=5)
81-99
90
Heparin plasma (n=5)
80-89
84
Linearity:
The linearity of the kit was assayed by testing samples spiked with appropriate concentration of the index and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.
Sample
1:2
1:4
1:8
1:16
Serum (n=5)
82-96%
83-98%
81-99%
93-101%
EDTA plasma (n=5)
88-101%
86-95%
90-102%
80-93%
Heparin plasma (n=5)
80-91%
82-90%
95-104%
79-95%
Intra-assay Precision:
Intra-Assay: CV <10%. 3 samples with low, middle and high level the index were tested 20 times on one plate, respectively.
Inter-assay Precision:
Inter-Assay: CV <12%. 3 samples with low, middle and high level the index were tested on 3 different plates, 8 replicates in each plate.
Stability:
The stability of ELISA kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage conditions. Note: To minimize unnecessary influences on the performance, operation procedures and lab conditions, especially room temperature, air humidity and incubator temperatures should be strictly regulated. It is also strongly suggested that the whole assay is performed by the same experimenter from the beginning to the end.
Step
Protocol
1.
Prepare all reagents, samples and standards
2.
Add 100µL standard or sample to each well. Incubate 2 hours at 37°C
3.
Aspirate and add 100µL prepared Detection Reagent A. Incubate 1 hour at 37°C
4.
Aspirate and wash 3 times
5.
Add 100µL prepared Detection Reagent B. Incubate 1 hour at 37°C
6.
Aspirate and wash 5 times
7.
Add 90µL Substrate Solution. Incubate 15-25 minutes at 37°C
8.
Add 50µL Stop Solution. Read at 450nm immediately.
