The Rat TXB2 (Thromboxane B2) ELISA Kit is a reliable and accurate tool for the detection of thromboxane B2 levels in rat serum, plasma, and cell culture supernatants. With its high sensitivity and specificity, this kit provides trustworthy and consistent results, making it suitable for a variety of research applications.Thromboxane B2 is a metabolite of thromboxane A2, a potent vasoconstrictor and platelet aggregator. Elevated levels of thromboxane B2 have been associated with various conditions such as inflammation, cardiovascular diseases, and platelet disorders. By measuring thromboxane B2 levels using this ELISA kit, researchers can gain valuable insights into the role of thromboxane B2 in these conditions and explore potential therapeutic interventions.
Product Name:
Rat TXB2 (Thromboxane B2) ELISA Kit
SKU:
RTES00806
Size:
96 Assays
Detection Method:
Colorimetric method, ELISA, Competitive
Assay type:
Competitive-ELISA
Assay time:
2 h 30 min
Sensitivity:
9.38 pg/mL
Detection range:
15.63-1000 pg/mL
Reovery:
80%-120%
This ELISA kit uses the Competitive-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with the target antigen. Standards or samples are added along with a biotinylated detection antibody. The target antigen present in the sample competes with the immobilized antigen for binding to the detection antibody. After incubation, Avidin-Horseradish Peroxidase (HRP) conjugate is added. Free components are washed away. The substrate solution is then added, resulting in a color change. The intensity of the color is inversely proportional to the concentration of the target antigen in the sample. The reaction is stopped by the addition of stop solution, and the color changes from blue to yellow. The optical density (OD) is measured at 450 nm ± 2 nm. The concentration of the target protein is calculated by comparing the OD values of the samples to the standard curve.
