Anti-RBP3 Antibody (CAB6403)

Antibody Name:	Anti-RBP3 Antibody
Antibody SKU:	CAB6403
Antibody Size:	20uL, 50uL, 100uL
Application:	WB IHC IF
Reactivity:	Human, Mouse, Rat
Host Species:	Rabbit
Immunogen:	Recombinant fusion protein containing a sequence corresponding to amino acids 18-320 of human RBP3 (NP_002891.1).

Application:	WB IHC IF
Recommended Dilution:	WB 1:500 - 1:2000 IHC 1:50 - 1:100 IF 1:50 - 1:100
Reactivity:	Human, Mouse, Rat
Positive Samples:	Mouse lung, Mouse eye, Rat eye

Immunogen:	Recombinant fusion protein containing a sequence corresponding to amino acids 18-320 of human RBP3 (NP_002891.1).
Purification Method:	Affinity purification
Storage Buffer:	Store at -20'C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Isotype:	IgG
Sequence:	GPTH LFQP SLVL DMAK VLLD NYCF PENL LGMQ EAIQ QAIK SHEI LSIS DPQT LASV LTAG VQSS LNDP RLVI SYEP STPE PPPQ VPAL TSLS EEEL LAWL QRGL RHEV LEGN VGYL RVDS VPGQ EVLS MMGE FLVA HVWG NLMG TSAL VLDL RHCT GGQV SGIP YIIS YLHP GNTI LHVD TIYN RPSN TTTE IWTL PQVL GERY GADK DVVV LTSS QTRG VAED IAHI LKQM RRAI VVGE RTGG GALD LRKL RIGE SDFF FTVP VSRS LGPL GGGS QTWE GSGV LPCV GTPA EQAL EKAL AIL
Gene ID:	5949
Uniprot:	P10745
Cellular Location:	Secreted, extracellular matrix, extracellular space, interphotoreceptor matrix
Calculated MW:	135kDa
Observed MW:	180kDa

Synonyms:

RBP3, D10S64, D10S65, D10S66, IRBP, RBPI, RP66

Background:

Interphotoreceptor retinol-binding protein is a large glycoprotein known to bind retinoids and found primarily in the interphotoreceptor matrix of the retina between the retinal pigment epithelium and the photoreceptor cells. It is thought to transport retinoids between the retinal pigment epithelium and the photoreceptors, a critical role in the visual process.The human IRBP gene is approximately 9.5 kbp in length and consists of four exons separated by three introns. The introns are 1.6-1.9 kbp long. The gene is transcribed by photoreceptor and retinoblastoma cells into an approximately 4.3-kilobase mRNA that is translated and processed into a glycosylated protein of 135, 000 Da. The amino acid sequence of human IRBP can be divided into four contiguous homology domains with 33-38% identity, suggesting a series of gene duplication events. In the gene, the boundaries of these domains are not defined by exon-intron junctions, as might have been expected. The first three homology domains and part of the fourth are all encoded by the first large exon, which is 3, 180 base pairs long. The remainder of the fourth domain is encoded in the last three exons, which are 191, 143, and approximately 740 base pairs long, respectively.

UniProt Protein Function:	RBP3: IRBP shuttles 11-cis and all trans retinoids between the retinol isomerase in the pigment epithelium and the visual pigments in the photoreceptor cells of the retina. Belongs to the peptidase S41A family.
UniProt Protein Details:	Protein type:Secreted, signal peptide; Secreted Chromosomal Location of Human Ortholog: 10q11.2 Cellular Component: extracellular space; extracellular region; vesicle Molecular Function:retinoid binding; serine-type peptidase activity; retinol binding; retinal binding Biological Process: phototransduction, visible light; visual perception; transport; lipid metabolic process; proteolysis; retinoid metabolic process Disease: Retinitis Pigmentosa 66; Retinitis Pigmentosa
NCBI Summary:	Interphotoreceptor retinol-binding protein is a large glycoprotein known to bind retinoids and found primarily in the interphotoreceptor matrix of the retina between the retinal pigment epithelium and the photoreceptor cells. It is thought to transport retinoids between the retinal pigment epithelium and the photoreceptors, a critical role in the visual process.The human IRBP gene is approximately 9.5 kbp in length and consists of four exons separated by three introns. The introns are 1.6-1.9 kbp long. The gene is transcribed by photoreceptor and retinoblastoma cells into an approximately 4.3-kilobase mRNA that is translated and processed into a glycosylated protein of 135,000 Da. The amino acid sequence of human IRBP can be divided into four contiguous homology domains with 33-38% identity, suggesting a series of gene duplication events. In the gene, the boundaries of these domains are not defined by exon-intron junctions, as might have been expected. The first three homology domains and part of the fourth are all encoded by the first large exon, which is 3,180 base pairs long. The remainder of the fourth domain is encoded in the last three exons, which are 191, 143, and approximately 740 base pairs long, respectively. [provided by RefSeq, Jul 2008]
UniProt Code:	P10745
NCBI GenInfo Identifier:	124894
NCBI Gene ID:	5949
NCBI Accession:	P10745.2
UniProt Secondary Accession:	P10745,Q0QD34, Q5VSR0, Q8IXN0,
UniProt Related Accession:	P10745
Molecular Weight:	1247
NCBI Full Name:	Retinol-binding protein 3
NCBI Synonym Full Names:	retinol binding protein 3, interstitial
NCBI Official Symbol:	RBP3
NCBI Official Synonym Symbols:	IRBP; RBPI; RP66; D10S64; D10S65; D10S66
NCBI Protein Information:	retinol-binding protein 3; interphotoreceptor retinoid-binding protein
UniProt Protein Name:	Retinol-binding protein 3
UniProt Synonym Protein Names:	Interphotoreceptor retinoid-binding protein; IRBP; Interstitial retinol-binding protein
Protein Family:	Retinol-binding protein
UniProt Gene Name:	RBP3
UniProt Entry Name:	RET3_HUMAN

	Western blot analysis of extracts of various cell lines, using RBP3 antibody (CAB6403) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 90s.
	Immunofluorescence analysis of MCF-7 cells using RBP3 antibody (CAB6403). Blue: DAPI for nuclear staining.

Antibodies	ELISA Kits
RBP3 Antibody (PACO11769)	Human RBP3 (Retinol Binding Protein 3, Interstitial) CLIA Kit (HUES00901)

Secondary Antibody

Anti-HRP Goat Anti-Rabbit IgG (H+L) Antibody (CABS014)