Anti-RBPJ Antibody (CAB5675)

Antibody Name:	Anti-RBPJ Antibody
Antibody SKU:	CAB5675
Antibody Size:	20uL, 50uL, 100uL
Application:	WB IHC IF
Reactivity:	Human, Mouse, Rat
Host Species:	Rabbit
Immunogen:	Recombinant fusion protein containing a sequence corresponding to amino acids 1-260 of human RBPJ (NP_976028.1).

Application:	WB IHC IF
Recommended Dilution:	WB 1:500 - 1:2000 IHC 1:50 - 1:200 IF 1:20 - 1:50
Reactivity:	Human, Mouse, Rat
Positive Samples:	Mouse liver

Immunogen:	Recombinant fusion protein containing a sequence corresponding to amino acids 1-260 of human RBPJ (NP_976028.1).
Purification Method:	Affinity purification
Storage Buffer:	Store at -20'C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Isotype:	IgG
Sequence:	MGGC RKFG ERPP PKRL TREA MRNY LKER GDQT VLIL HAKV AQKS YGNE KRFF CPPP CVYL MGSG WKKK KEQM ERDG CSEQ ESQP CAFI GIGN SDQE MQQL NLEG KNYC TAKT LYIS DSDK RKHF MLSV KMFY GNSD DIGV FLSK RIKV ISKP SKKK QSLK NADL CIAS GTKV ALFN RLRS QTVS TRYL HVEG GNFH ASSQ QWGA FFIH LLDD DESE GEEF TVRD GYIH YGQT VKLV CSVT GMAL PRLI IRKV DKQT ALLD
Gene ID:	3516
Uniprot:	Q06330
Cellular Location:	Cytoplasm, Nucleus
Calculated MW:	45kDa/47kDa/51kDa/54kDa/55kDa
Observed MW:	61KDa

Synonyms:

RBPJ, AOS3, CBF1, IGKJRB, IGKJRB1, KBF2, RBP-J, RBPJK, RBPSUH, SUH, csl

Background:

The protein encoded by this gene is a transcriptional regulator important in the Notch signaling pathway. The encoded protein acts as a repressor when not bound to Notch proteins and an activator when bound to Notch proteins. It is thought to function by recruiting chromatin remodeling complexes containing histone deacetylase or histone acetylase proteins to Notch signaling pathway genes. Several transcript variants encoding different isoforms have been found for this gene, and several pseudogenes of this gene exist on chromosome 9.

UniProt Protein Function:	RBPJ: Transcriptional regulator that plays a central role in Notch signaling, a signaling pathway involved in cell-cell communication that regulates a broad spectrum of cell-fate determinations. Acts as a transcriptional repressor when it is not associated with Notch proteins. When associated with some NICD product of Notch proteins (Notch intracellular domain), it acts as a transcriptional activator that activates transcription of Notch target genes. Probably represses or activates transcription via the recruitment of chromatin remodeling complexes containing histone deacetylase or histone acetylase proteins, respectively. Specifically binds to the immunoglobulin kappa-type J segment recombination signal sequence. Binds specifically to methylated DNA. Interacts with activated NOTCH1, NOTCH2 or NOTCH3. Interacts with MINT/SHARP. This interaction may mediate the recruitment of large corepressor complexes containing proteins such as HDAC1, HDAC2, NCOR2, SAP30, FHL1/KYOT2 and CIR1. Interacts with EP300, MAML1 and PTF1A. Interacts with Epstein-Barr virus EBNA2, EBNA3, EBNA4 and EBNA6. Interacts with RITA/C12orf52, leading to nuclear export, prevent the interaction between RBPJ and NICD product and subsequent down-regulation of the Notch signaling pathway. Belongs to the Su(H) family. 7 isoforms of the human protein are produced by alternative splicing.
UniProt Protein Details:	Protein type:Transcription factor; Cell development/differentiation Chromosomal Location of Human Ortholog: 4p15.2 Cellular Component: nucleoplasm; transcription factor complex; cytoplasm; nucleolus; nucleus Molecular Function:protein binding; DNA binding; recombinase activity; protein N-terminus binding; chromatin binding; transcription factor activity Biological Process: somatic stem cell maintenance; myeloid dendritic cell differentiation; sebaceous gland development; negative regulation of transcription from RNA polymerase II promoter; DNA recombination; negative regulation of cell proliferation; negative regulation of ossification; angiogenesis; regulation of timing of cell differentiation; positive regulation of BMP signaling pathway; positive regulation of cardiac muscle cell proliferation; transcription initiation from RNA polymerase II promoter; epidermal cell fate specification; Notch signaling pathway; hair follicle maturation; humoral immune response; activation of Notch receptor target transcription factor; keratinocyte differentiation; inflammatory response to antigenic stimulus; pituitary gland development; B cell differentiation; defense response to bacterium; epithelial to mesenchymal transition; gene expression; positive regulation of transcription from RNA polymerase II promoter; blood vessel remodeling; auditory receptor cell fate commitment; negative regulation of transcription, DNA-dependent Disease: Adams-oliver Syndrome 3
NCBI Summary:	The protein encoded by this gene is a transcriptional regulator important in the Notch signaling pathway. The encoded protein acts as a repressor when not bound to Notch proteins and an activator when bound to Notch proteins. It is thought to function by recruiting chromatin remodeling complexes containing histone deacetylase or histone acetylase proteins to Notch signaling pathway genes. Several transcript variants encoding different isoforms have been found for this gene, and several pseudogenes of this gene exist on chromosome 9. [provided by RefSeq, Oct 2013]
UniProt Code:	Q06330
NCBI GenInfo Identifier:	338817983
NCBI Gene ID:	3516
NCBI Accession:	Q06330.3
UniProt Secondary Accession:	Q06330,Q5XKH9, Q6P1N3, B4DY22,
UniProt Related Accession:	Q06330
Molecular Weight:	500
NCBI Full Name:	Recombining binding protein suppressor of hairless
NCBI Synonym Full Names:	recombination signal binding protein for immunoglobulin kappa J region
NCBI Official Symbol:	RBPJ
NCBI Official Synonym Symbols:	SUH; csl; AOS3; CBF1; KBF2; RBP-J; RBPJK; IGKJRB; RBPSUH; IGKJRB1
NCBI Protein Information:	recombining binding protein suppressor of hairless; CBF-1; RBP-JK; RBP-J kappa; H-2K binding factor-2; suppressor of hairless homolog; renal carcinoma antigen NY-REN-30; immunoglobulin kappa J region recombination signal binding protein 1
UniProt Protein Name:	Recombining binding protein suppressor of hairless
UniProt Synonym Protein Names:	CBF-1; J kappa-recombination signal-binding protein; RBP-J kappa; RBP-J; RBP-JK; Renal carcinoma antigen NY-REN-30
Protein Family:	RBPJ-interacting and tubulin-associated protein
UniProt Gene Name:	RBPJ
UniProt Entry Name:	SUH_HUMAN

	Western blot analysis of extracts of Mouse liver, using RBPJ Rabbit pAb (CAB5675) at 1:500 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 180s.
	Immunohistochemistry of paraffin-embedded rat brain using RBPJ Antibody (CAB5675) at dilution of 1:100 (40x lens).
	Immunohistochemistry of paraffin-embedded human stomach using RBPJ Antibody (CAB5675) at dilution of 1:100 (40x lens).
	Immunofluorescence analysis of U2OS cells using RBPJ antibody (CAB5675). Blue: DAPI for nuclear staining.

Antibodies	ELISA Kits
Anti-RBPJK Antibody (CAB4081)	Human RBPJ (Recombining binding protein suppressor of hairless) ELISA Kit (HUFI08556)
RBPJ Antibody (PACO11773)

Secondary Antibody

Anti-HRP Goat Anti-Rabbit IgG (H+L) Antibody (CABS014)