Recombinant 2019-nCoV Nucleocapsid Protein, Biotinylated (His Tag)

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SKU:
RPES0057
€980

Description

Product Background

Coronaviruses are enveloped viruses with a positive-sense RNA genome and with a nucleocapsid of helical symmetry. Coronavirus nucleoproteins localize to the cytoplasm and the nucleolus, a subnuclear structure, in both virus-infected primary cells and in cells transfected with plasmids that express N protein. Coronavirus N protein is required for coronavirus RNA synthesis, and has RNA chaperone activity that may be involved in template switch. Nucleocapsid protein is a most abundant protein of coronavirus. During virion assembly, N protein binds to viral RNA and leads to formation of the helical nucleocapsid. Nucleocapsid protein is a highly immunogenic phosphoprotein also implicated in viral genome replication and in modulating cell signaling pathways. Because of the conservation of N protein sequence and its strong immunogenicity, the N protein of coronavirus is chosen as a diagnostic tool.

Key Information Description

Product Name

Recombinant 2019-nCoV Nucleocapsid Protein, Biotinylated (His Tag)

Size

20µg

SKU

RPES0057

Synonyms

NP;2019-nCoV coronavirus NP Protein; 2019-nCoV np Protein; 2019-nCoV novel coronavirus Nucleoprotein Protein

Species

Virus

Expression host

Baculovirus-Insect Cells

Sequence

(Met1-Ala419(335Gly/Ala))

Accession

YP_009724397.2

Molar Mass

47.08 kDa

Tag

C-His

Purity

> 85 % as determined by reducing SDS-PAGE.

Endotoxin

< 1.0 EU per µg as determined by the LAL method.

Storage

Lyophilized proteins are stable for up to 12 months when stored at -20 to -80°C. Reconstituted protein solution can be stored at 4-8℃ for 2-7 days. Aliquots of reconstituted samples are stable at < -20℃ for 3 months.

Shipping

This product is provided as lyophilized powder which is shipped with ice packs.

Formulation

Lyophilized from sterile PBS, 500 mM NaCl, pH 7.0.

Reconstitution

Please refer to the printed manual for detailed information.

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