The Recombinant 4E-BP1/EIF4EBP1 Monoclonal Antibody is a recombinant rabbit monoclonal antibody (clone C110038) against EIF4EBP1. This gene encodes one member of a family of translation repressor proteins. The protein directly interacts with eukaryotic translation initiation factor 4E (eIF4E), which is a limiting component of the multisubunit complex that recruits 40S ribosomal subunits to the 5' end of mRNAs. Interaction of this protein with eIF4E inhibits complex assembly and represses translation. Produced by recombinant technology in Rabbit hosts and purified by Protein A affinity chromatography, this Rabbit IgG antibody is reactive against Human, Mouse and Rat samples.
This antibody is suitable for WB, IHC-P, ICC/IF, FC and IP applications. It has been validated by WB analysis of K-562, A431, HL-60, HeLa, RAW264.7, C6, IHC analysis of Human colon cancer and IF analysis of Hela. The target is localized to the cytoplasm, cytosol.
Product Name:
Recombinant 4E-BP1/EIF4EBP1 Monoclonal Antibody
SKU:
RMEA0052
Size:
50 µL, 100 µL
Antibody Type:
Recombinant Monoclonal Antibody
Synonyms:
4E-BP, 4E-BP1, 4EBP, 4EBP1, BP 1, BP-1, eIF4E binding protein, eIF4E binding protein 1, eIF4E-binding protein 1, EIF4EBP, EIF4EBP1, eukaryotic translation initiation factor 4E-binding protein, eukaryotic translation initiation factor 4E-binding protein 1, PHAS-I, PHASI, Phosphorylated heat- and acid-stable protein regulated by insulin 1
Store at -20°C valid for 12 months. Avoid freeze / thaw cycles.
Immunofluorescence analysis of HeLa cells labelling 4E BP1. Nuclear DNA was labeled in blue with DAPI. Control: Secondary antibody only.
Immunohistochemical analysis of paraffin-embedded Human colon cancer tissue sections using 4E- BP1/EIF4EBP1 Antibody (RMEA0052) at a dilution of 1:150 (10× lens). Heat mediated antigen retrieval was performed with Tris-EDTA buffer (pH 9.0) for 20 min prior to IHC staining.
Western blot analysis of K-562,A431,HL-60,HeLa,RAW264.7 and C6 cell lysates using 4E-BP1/EIF4EBP1 Antibody (RMEA0052) at a dilution of 1:1000. Observed-MW:15-20 kDa Calculated-MW:13 kDa
