The Recombinant alpha Actinin 2 Monoclonal Antibody is a recombinant rabbit monoclonal antibody (clone C111377) against alpha Actinin 2. ACTN2 (Actinin Alpha 2) is a Protein Coding gene. This gene encodes a muscle-specific, alpha actinin isoform that is expressed in both skeletal and cardiac muscles. Among its related pathways are RET signaling and Striated Muscle Contraction. Produced by recombinant technology in Rabbit hosts and purified by Protein A affinity chromatography, this Rabbit IgG antibody is reactive against Human, Mouse and Rat samples.
This antibody is suitable for WB, IHC and IF applications. It has been validated by WB analysis of Mouse heart, Rat heart, Mouse brain, Rat brain, IHC analysis of Human cardiac muscle, Human skeletal muscle, Mouse cardiac muscle, Rat cardiac muscle and IF analysis of HeLa. The target is localized to the cytoplasm.
PBS, 50% glycerol, 0.05% Proclin 300, 0.05% protein protectant.
Storage:
Store at -20°C valid for 12 months. Avoid freeze / thaw cycles.
Immunofluorescent analysis of (4% PFA) fixed HeLa cells using anti-alpha Actinin 2 Monoclonal Antibody at dilution of 1:50.
Immunohistochemistry of paraffin-embedded Human cardiac muscle using alpha Actinin 2 Monoclonal Antibody at dilution of 1:500.
Immunohistochemistry of paraffin-embedded Human skeletal muscle using alpha Actinin 2 Monoclonal Antibody at dilution of 1:500.
Immunohistochemistry of paraffin-embedded Mouse cardiac muscle using alpha Actinin 2 Monoclonal Antibody at dilution of 1:100.
Immunohistochemistry of paraffin-embedded Rat cardiac muscle using alpha Actinin 2 Monoclonal Antibody at dilution of 1:500.
Western Blot with alpha Actinin 2 Monoclonal Antibody at dilution of 1:10000. Lane 1: Mouse heart Observed-MW:103 kDa Calculated-MW:103 kDa
Western Blot with alpha Actinin 2 Monoclonal Antibody at dilution of 1:10000. Lane 1: Rat heart, Lane 2: Mouse brain, Lane 3: Rat brain Observed-MW:103 kDa Calculated-MW:103 kDa
