The Recombinant beta-2 Adrenergic Receptor Monoclonal Antibody is a recombinant rabbit monoclonal antibody (clone C171068) against beta-2 Adrenergic Receptor. Beta-adrenergic receptors mediate the catecholamine-induced activation of adenylate cyclase through the action of G proteins. The beta-2-adrenergic receptor binds epinephrine with an approximately 30-fold greater affinity than it does norepinephrine. Produced by recombinant technology in Rabbit hosts and purified by Protein A affinity chromatography, this Rabbit IgG antibody is reactive against Human, Mouse and Rat samples.
This antibody is suitable for WB, IF and FCM applications. It has been validated by WB analysis of A431, MCF-7, MDA-MB-231, Rat kidney, Mouse kidney, Mouse kidney, IF analysis of A431, MCF7 and FCM analysis of SH-SY5Y. The target is localized to the endosome, plasm membrane, Golgi apparatus.
Recombinant human beta-2 Adrenergic Receptor fragment
Applications:
WBIFFCM
Clonality:
Recombinant Monoclonal
Clone No:
C171068
Host:
Rabbit
Isotype:
Rabbit IgG
Target:
beta-2 Adrenergic Receptor
Conjugation:
Unconjugated
Observed MW:
46 kDa
Calculated MW:
46 kDa
Recommended Dilution:
WB 1:500-2000; IF 1:100; FCM 1:50-100
UniProt:
P07550
Purification:
Protein A
Concentration:
1 mg/mL
Buffer:
PBS, 50% glycerol, 0.05% Proclin 300, 0.05% protein protectant.
Storage:
Store at -20°C valid for 12 months. Avoid freeze / thaw cycles.
Flow cytometric analysis of human beta-2 Adrenergic Receptor expression on SH-SY5Y cells. Cells were stained with purified anti-Human beta-2 Adrenergic Receptor, then a Alexa Fluor 488-conjugated second step antibody. The histogram were derived from events with the forward and side light-scatter characteristics of intact cells.
Immunofluorescent analysis of (100% Ice-cold methanol) fixed A431 cells using anti-beta-2 Adrenergic Receptor Monoclonal Antibody at dilution of 1:100.
Immunofluorescent analysis of (100% Ice-cold methanol) fixed MCF7 cells using anti-beta-2 Adrenergic Receptor Monoclonal Antibody at dilution of 1:100.
Western Blot with beta-2 Adrenergic Receptor Monoclonal Antibody at dilution of 1:2000. Lane 1: A431, Lane 2: MCF-7, Lane 3: MDA-MB-231, Lane 4: Rat kidney, Lane 5: Mouse kidney Observed-MW:46 kDa Calculated-MW:46 kDa
Western Blot with beta-2 Adrenergic Receptor Monoclonal Antibody at dilution of 1:1000. Lane 1: Mouse kidney Observed-MW:46 kDa Calculated-MW:46 kDa
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