The Recombinant beta Catenin Monoclonal Antibody is a recombinant rabbit monoclonal antibody (clone C123231) against beta Catenin. CTNNB1, also known as β-Catenin, is an evolutionarily conserved, multifunctional intracellular protein. CTNNB1 was originally identified in cell adherens junctions (Ajs) where it functions to bridge the cytoplasmic domain of cadherins to a-catenin and the actin cytoskeleton. Besides its essential role in the Ajs, CTNNB1 is also a key downstream component of the canonical Wnt pathway that plays diverse and critical roles in embryonic development and adult tissue homeostasis. Produced by recombinant technology in Rabbit hosts and purified by affinity chromatography, this Rabbit IgG antibody is reactive against Human, Mouse, Rat and Hamster samples.
This antibody is suitable for WB and IHC-P applications. It has been validated by WB analysis of C6, CHO-K1, Hela and IHC analysis of Human Cholangiocarcinoma. The target is localized to the Cytoplasm. Nucleus. Cytoplasm>cytoskeleton. Cell junction>adherens junction. Cell junction. Cell membrane. Cytoplasmic when it is unstabilized (high level of phosphorylation) or bound to CDH1. Translocates to the nucleus when it is stabilized (low level of phosphorylation). Interaction with GLIS2 and MUC1 promotes nuclear translocation. Interaction with EMD inhibits nuclear localization.
