The Recombinant β-Galactosidase Monoclonal Antibody is a recombinant rabbit monoclonal antibody (clone C110529) against β-Galactosidase. This gene encodes a member of the glycosyl hydrolase 35 family of proteins. Alternative splicing results in multiple transcript variants, at least one of which encodes a preproprotein that is proteolytically processed to generate the mature lysosomal enzyme. This enzyme catalyzes the hydrolysis of a terminal beta-linked galactose residue from ganglioside substrates and other glycoconjugates. Produced by recombinant technology in Rabbit hosts and purified by Protein A affinity chromatography, this Rabbit IgG antibody is reactive against Human, Mouse and Rat samples.
This antibody is suitable for WB, IHC, IF and ELISA applications. It has been validated by WB analysis of Mouse brain. The target is localized to the lysosome.
IHC 1:2000-10000; WB 1:1000-5000; IF 1:200-1000; ELISA 1:5000-20000
UniProt:
P16278
Purification:
Protein A
Concentration:
0.2 mg/mL
Buffer:
PBS, 50% glycerol, 0.05% Proclin 300, 0.05% protein protectant.
Storage:
Store at -20°C valid for 12 months. Avoid freeze / thaw cycles.
Western Blot with Recombinant β-Galactosidase Monoclonal Antibody at dilution of 1:1000 dilution. Lane A: Mouse brain cells. Observed-MW:100 kDa Calculated-MW:76 kDa
