The Recombinant BMI1 Monoclonal Antibody is a recombinant rabbit monoclonal antibody (clone C110101) against BMI1. This gene encodes a ring finger protein that is major component of the polycomb group complex 1 (PRC1). This complex functions through chromatin remodeling as an essential epigenetic repressor of multiple regulatory genes involved in embryonic development and self-renewal in somatic stem cells. This protein also plays a central role in DNA damage repair. Produced by recombinant technology in Rabbit hosts and purified by Protein A affinity chromatography, this Rabbit IgG antibody is reactive against Human, Mouse and Rat samples.
This antibody is suitable for WB, IHC, IF, IP and ELISA applications. It has been validated by WB analysis of Ramos, C6, Mouse brain and IHC analysis of Human tonsil. The target is localized to the nucleus, cytoplasm.
A synthetic peptide corresponding to the center region of the Human BMI1
Applications:
WBIHCIFIPELISA
Clonality:
Recombinant Monoclonal
Clone No:
C110101
Host:
Rabbit
Isotype:
Rabbit IgG
Target:
BMI1
Conjugation:
Unconjugated
Observed MW:
43 kDa
Calculated MW:
37 kDa
Recommended Dilution:
IHC 1:100-500; WB 1:2000-10000; IF 1:200-1000; ELISA 1:5000-20000; IP 1:50-200
UniProt:
P35226, P25916
Purification:
Protein A
Concentration:
1 mg/mL
Buffer:
0.2 µm filtered solution in PBS
Storage:
Store at -20°C valid for 12 months. Avoid freeze / thaw cycles.
Human tonsil was stained with anti-Bmi-1 Rabbit antibody
Various whole cell lysates were separated by 4-20% SDS- PAGE, and the membrane was blotted with anti-Bmi-1 antibody. The HRP-conjugated Goat anti-Rabbit IgG (H + L) antibody was used to detect the antibody. Lane 1: Ramos Lane 2: C6 Lane 3: Mouse brain Predicted band size: 37kDa Observed band size: 43kDa