The Recombinant BMP2 Monoclonal Antibody is a recombinant rabbit monoclonal antibody (clone C111742) against BMP2. Bone morphogenetic protein-2(BMP-2) is a menmber of the transforming growth factor-beta (TGFB) superfamily. BMP2 is synthesized as a 60 kDa precursor that is processed in the secretory pathway to a small 18 kDa monomer, 2 monomersthen associate to form the active 30 kDa homodimer, which binds to its receptor. There is also a 40-45 kDa form of BMP2, as an amino-teminal propeptide. Produced by recombinant technology in Rabbit hosts and purified by Protein A affinity chromatography, this Rabbit IgG antibody is reactive against Human, Mouse and Rat samples.
This antibody is suitable for WB, IHC, IF and ELISA applications. It has been validated by WB analysis of Hela, MCF7, RAW264.7, Rat spleen, IHC analysis of Rat spine and IF analysis of HEK293. The target is localized to the secreted.
Product Name:
Recombinant BMP2 Monoclonal Antibody
SKU:
AGEL7047
Size:
50 µL, 100 µL
Antibody Type:
Recombinant Monoclonal Antibody
Synonyms:
BDA, BDA2, BDA2A, BMP, BMP-2, BMP-2A, BMP2, BMP2A, Bone morphogenetic protein 2, Bone morphogenetic protein 2A
Reactivity:
Human, Mouse, Rat
Immunogen:
Recombinant protein corresponding to Mouse BMP2
Applications:
WBIHCIFELISA
Clonality:
Recombinant Monoclonal
Clone No:
C111742
Host:
Rabbit
Isotype:
Rabbit IgG
Target:
BMP2
Conjugation:
Unconjugated
Observed MW:
30, 13 kDa
Calculated MW:
45, 13 kDa
Recommended Dilution:
IHC 1:200-1000; WB 1:1000-5000; IF 1:200-1000; ELISA 1:5000-2000
UniProt:
P12643, P21274, P49001
Purification:
Protein A
Concentration:
1 mg/mL
Buffer:
PBS, 50% glycerol, 0.05% Proclin 300, 0.05% protein protectant.
Storage:
Store at -20°C valid for 12 months. Avoid freeze / thaw cycles.
Immunofluorescence analysis of HEK293. Picture A: BMP2 antibody (red), Picture B: DAPI (blue), Picture C: Merge of A+B
Rat spine was stained with anti-BMP2 Rabbit antibody.
Lane 1: Hela, Lane 2: MCF7, Lane 3: RAW264.7, Lane 4: Rat spleen