The Recombinant Casein Kinase 2 beta Monoclonal Antibody is a recombinant rabbit monoclonal antibody (clone C111442) against Casein Kinase 2 beta. Casein Kinase 2 beta is a ubiquitous protein kinase which regulates metabolic pathways, signal transduction, transcription, translation, and replication. The enzyme is composed of three subunits, alpha, alpha prime and beta, which form a tetrameric holoenzyme. The alpha and alpha prime subunits are catalytic, while the beta subunit serves regulatory functions. Produced by recombinant technology in Rabbit hosts and purified by Protein A affinity chromatography, this Rabbit IgG antibody is reactive against Human, Mouse and Rat samples.
This antibody is suitable for WB, IHC and IF applications. It has been validated by WB analysis of HeLa, MCF-7, NIH/3T3, C6, IHC analysis of Human colon cancer, Rat cerebrum and IF analysis of NIH/3T3. The target is localized to the cytoplasm, nucleus, secreted.
casein kinase 2 beta, Casein kinase 2 beta polypeptide, Casein kinase II beta subunit, Casein kinase II subunit beta, CK II beta, CK2B, CK2N, Ckb, Ckb1, Ckb2, CSK2B, CSNK 2B, CSNK2B, G5A, MGC138222, MGC138224, Phosvitin, Protein G5a
Reactivity:
Human, Mouse, Rat
Immunogen:
Recombinant human Casein Kinase 2 beta fragment
Applications:
WBIHCIF
Clonality:
Recombinant Monoclonal
Clone No:
C111442
Host:
Rabbit
Isotype:
Rabbit IgG
Target:
Casein Kinase 2 beta
Conjugation:
Unconjugated
Observed MW:
25 kDa
Calculated MW:
25 kDa
Recommended Dilution:
WB 1:500-1000; IHC 1:50-100; IF 1:50
UniProt:
P67870
Purification:
Protein A
Concentration:
1 mg/mL
Buffer:
PBS, 50% glycerol, 0.05% Proclin 300, 0.05% protein protectant.
Storage:
Store at -20°C valid for 12 months. Avoid freeze / thaw cycles.
Immunofluorescent analysis of (100% Ice-cold methanol) fixed NIH/3T3 cells using anti-Casein Kinase 2 beta Monoclonal Antibody at dilution of 1:50.
Immunohistochemistry of paraffin-embedded Human colon cancer using Casein Kinase 2 beta Monoclonal Antibody at dilution of 1:100.
Immunohistochemistry of paraffin-embedded Rat cerebrum using Casein Kinase 2 beta Monoclonal Antibody at dilution of 1:100.
Western Blot with Casein Kinase 2 beta Monoclonal Antibody at dilution of 1:1000. Lane 1: HeLa, Lane 2: MCF-7, Lane 3: NIH/3T3, Lane 4: C6 Observed-MW:25 kDa Calculated-MW:25 kDa