The Recombinant Caspase-7 Monoclonal Antibody is a recombinant rabbit monoclonal antibody (clone C221079) against Caspase-7. Involved in the activation cascade of caspases responsible for apoptosis execution. Cleaves and activates sterol regulatory element binding proteins (SREBPs). Proteolytically cleaves poly(ADP-ribose) polymerase (PARP) at a '216-Asp-|-Gly-217' bond. Produced by recombinant technology in Rabbit hosts and purified by Protein A affinity chromatography, this Rabbit IgG antibody is reactive against Human and Mouse samples.
This antibody is suitable for WB and IHC applications. It has been validated by WB analysis of HeLa, RAW264.7, Jurkat and IHC analysis of Human breast cancer, Human colon, Human liver cancer. The target is localized to the cytoplasm.
PBS, 50% glycerol, 0.05% Proclin 300, 0.05% protein protectant.
Storage:
Store at -20°C valid for 12 months. Avoid freeze / thaw cycles.
Immunohistochemistry of paraffin-embedded Human breast cancer using Caspase-7 Monoclonal Antibody at dilution of 1:1000.
Immunohistochemistry of paraffin-embedded Human colon using Caspase-7 Monoclonal Antibody at dilution of 1:1000.
Immunohistochemistry of paraffin-embedded Human liver cancer using Caspase-7 Monoclonal Antibody at dilution of 1:1000.
Western Blot with Caspase-7 Monoclonal Antibody at dilution of 1:2000. Lane 1: HeLa, Lane 2: RAW264.7, Lane 3: Jurkat Observed-MW:18, 32, 35 kDa Calculated-MW:34 kDa
