The Recombinant CD11b Monoclonal Antibody is a recombinant rabbit monoclonal antibody (clone C110421) against CD11b. This gene encodes the integrin alpha M chain. Integrins are heterodimeric integral membrane proteins composed of an alpha chain and a beta chain. This I-domain containing alpha integrin combines with the beta 2 chain (ITGB2) to form a leukocyte-specific integrin referred to as macrophage receptor 1 ('Mac-1'), or inactivated-C3b (iC3b) receptor 3 ('CR3'). Produced by recombinant technology in Rabbit hosts and purified by Protein A affinity chromatography, this Rabbit IgG antibody is reactive against Human, Mouse and Rat samples.
This antibody is suitable for WB, IHC, IF and ELISA applications. It has been validated by WB analysis of Mouse spleen. The target is localized to the membrane.
IHC 1:4000-20000; WB 1:2000-10000; IF 1:200-1000; ELISA 1:5000-20000
UniProt:
P11215
Purification:
Protein A
Concentration:
0.2 mg/mL
Buffer:
PBS, 50% glycerol, 0.05% Proclin 300, 0.05% protein protectant.
Storage:
Store at -20°C valid for 12 months. Avoid freeze / thaw cycles.
Western Blot with Recombinant CD11b Monoclonal Antibody at dilution of 1:1000 dilution. Lane A: Mouse spleen whole cell lysate. Observed-MW:170 kDa Calculated-MW:127 kDa
