The Recombinant CD200 Monoclonal Antibody is a recombinant rabbit monoclonal antibody (clone C320140) against CD200. This gene encodes a type I membrane glycoprotein containing two extracellular immunoglobulin domains, a transmembrane and a cytoplasmic domain. This gene is expressed by various cell types, including B cells, a subset of T cells, thymocytes, endothelial cells, and neurons. The encoded protein plays an important role in immunosuppression and regulation of anti-tumor activity. Produced by recombinant technology in Rabbit hosts and purified by Protein A affinity chromatography, this Rabbit IgG antibody is reactive against Human samples.
This antibody is suitable for WB and IHC-P applications. It has been validated by WB analysis of SH-SY5Y, HT-29 and IHC analysis of Human tonsil. The target is localized to the cell membrane.
Store at -20°C valid for 12 months. Avoid freeze / thaw cycles.
Immunohistochemical analysis of paraffin-embedded Human tonsil tissue sections using CD200 Antibody (RMEA0154) at a dilution of 1:300 (10× lens). Heat mediated antigen retrieval was performed with Tris-EDTA buffer (pH 9.0) for 20 min prior to IHC staining.
Western blot analysis of SH-SY5Y and HT-29 cell lysates using CD200 Antibody (RMEA0154) at a dilution of 1:1000. Observed-MW:45 kDa Calculated-MW:31 kDa