The Recombinant CD25 Monoclonal Antibody is a recombinant rabbit monoclonal antibody (clone C361095) against CD25. The interleukin 2 (IL2) receptor alpha (IL2RA) and beta (IL2RB) chains, together with the common gamma chain (IL2RG), constitute the high-affinity IL2 receptor. Homodimeric alpha chains (IL2RA) result in low-affinity receptor, while homodimeric beta (IL2RB) chains produce a medium-affinity receptor. Normally an integral-membrane protein, soluble IL2RA has been isolated and determined to result from extracellular proteolyisis. Produced by recombinant technology in Rabbit hosts and purified by Protein A affinity chromatography, this Rabbit IgG antibody is reactive against Human samples.
This antibody is suitable for IF and FCM applications. It has been validated by IF analysis of Jurkat, K562 and FCM analysis of Jurkat. The target is localized to the membrane.
PBS, 50% glycerol, 0.05% Proclin 300, 0.05% protein protectant.
Storage:
Store at -20°C valid for 12 months. Avoid freeze / thaw cycles.
Flow cytometric analysis of human CD25 expression on Jurkat cells. Cells were stained with purified anti-Human CD25, then a Alexa Fluor 488-conjugated second step antibody. The histogram were derived from events with the forward and side light-scatter characteristics of intact cells.
Immunofluorescent analysis of (4% Paraformaldehyde) fixed Jurkat cells using anti-CD25 Monoclonal Antibody at dilution of 1:50.
Immunofluorescent analysis of (4% Paraformaldehyde) fixed K562 cells using anti-CD25 Monoclonal Antibody at dilution of 1:50.
