The Recombinant CD27/TNFRSF7 Monoclonal Antibody is a recombinant rabbit monoclonal antibody (clone C110008) against CD27. The protein encoded by this gene is a member of the TNF-receptor superfamily. This receptor is required for generation and long-term maintenance of T cell immunity. It binds to ligand CD70, and plays a key role in regulating B-cell activation and immunoglobulin synthesis. Produced by recombinant technology in Rabbit hosts and purified by Protein A affinity chromatography, this Rabbit IgG antibody is reactive against Human, Mouse and Rat samples.
This antibody is suitable for WB, IHC, IF and ELISA applications. It has been validated by WB analysis of Ramos, Daudi, Raji and IHC analysis of Human tonsil. The target is localized to the membrane.
IHC 1:200-1000; WB 1:10000-50000; IF 1:200-1000; ELISA 1:5000-20000
UniProt:
P26842, P41272
Purification:
Protein A
Concentration:
1 mg/mL
Buffer:
0.2 µm filtered solution in PBS
Storage:
Store at -20°C valid for 12 months. Avoid freeze / thaw cycles.
Immunohistochemical analysis of paraffin-embedded Human tonsil tissue sections using CD27/TNFRSF7 Antibody (RMEA0022) at a dilution of 1:300 (10× lens). Heat mediated antigen retrieval was performed with Tris- EDTA buffer (pH 9.0) for 20 min prior to IHC staining.
Western blot analysis of Ramos and Raji cell lysates using CD27/TNFRSF7 Antibody (RMEA0022) at a dilution of 1:15000. Observed-MW:55kD Calculated-MW:29kD
