The Recombinant CD31 Monoclonal Antibody is a recombinant rabbit monoclonal antibody (clone C110535) against CD31. The protein encoded by this gene is found on the surface of platelets, monocytes, neutrophils, and some types of T-cells, and makes up a large portion of endothelial cell intercellular junctions. The encoded protein is a member of the immunoglobulin superfamily and is likely involved in leukocyte migration, angiogenesis, and integrin activation. Produced by recombinant technology in Rabbit hosts and purified by Protein A affinity chromatography, this Rabbit IgG antibody is reactive against Human, Mouse and Rat samples.
This antibody is suitable for WB, IHC, IF and ELISA applications. It has been validated by WB analysis of Jurkat, Mouse liver, Rat liver and IHC analysis of Human liver. The target is localized to the membrane.
IHC 1:1000-2000; WB 1:1000-5000; IF 1:200-1000; ELISA 1:5000-20000
UniProt:
P16284
Purification:
Protein A
Concentration:
0.2 mg/mL
Buffer:
PBS, 50% glycerol, 0.05% Proclin 300, 0.05% protein protectant.
Storage:
Store at -20°C valid for 12 months. Avoid freeze / thaw cycles.
Immunohistochemical analysis of paraffin-embedded Human liver tissue sections using CD31 Antibody (RMEA0544) at a dilution of 1:1500 (10× lens). Heat mediated antigen retrieval was performed with Tris-EDTA buffer (pH 9.0) for 20 min prior to IHC staining.
Western blot analysis of Jurkat,Mouse liver and Rat liver cell lysates using CD31 Antibody (RMEA0544) at a dilution of 1:1500. Observed-MW:130 kDa Calculated-MW:81 kDa
