The Recombinant CD8 α Monoclonal Antibody is a recombinant rabbit monoclonal antibody (clone C110707) against CD8 α. The CD8 antigen is a cell surface glycoprotein found on most cytotoxic T lymphocytes that mediates efficient cell-cell interactions within the immune system. The CD8 antigen acts as a coreceptor with the T-cell receptor on the T lymphocyte to recognize antigens displayed by an antigen presenting cell in the context of class I MHC molecules. The coreceptor functions as either a homodimer composed of two alpha chains or as a heterodimer composed of one alpha and one beta chain. Produced by recombinant technology in Rabbit hosts and purified by Protein A affinity chromatography, this Rabbit IgG antibody is reactive against Human, Mouse and Rat samples.
This antibody is suitable for WB, IHC, IF and ELISA applications. It has been validated by WB analysis of Mouse thymus and IHC analysis of Human colon tissue. The target is localized to the membranous.
IHC 1:200-1000; WB 1:1000-5000; IF 1:200-1000; ELISA 1:5000-20000
UniProt:
P01732
Purification:
Protein A
Concentration:
0.2 mg/mL
Buffer:
PBS, 50% glycerol, 0.05% Proclin 300, 0.05% protein protectant.
Storage:
Store at -20°C valid for 12 months. Avoid freeze / thaw cycles.
Immunohistochemistry of paraffin-embedded human colon tissue using Recombinant CD8 α Monoclonal Antibody at dilution of 1:200.
Western Blot with Recombinant CD8 α Monoclonal Antibody at dilution of 1:1000 dilution. Lane A: Mouse thymus cells. Observed-MW:35 kDa Calculated-MW:26 kDa
