The Recombinant CD81 Monoclonal Antibody is a recombinant rabbit monoclonal antibody (clone C110742) against CD81. The protein encoded by this gene is a member of the transmembrane 4 superfamily, also known as the tetraspanin family. Most of these members are cell-surface proteins that are characterized by the presence of four hydrophobic domains. The proteins mediate signal transduction events that play a role in the regulation of cell development, activation, growth and motility. Produced by recombinant technology in Rabbit hosts and purified by Protein A affinity chromatography, this Rabbit IgG antibody is reactive against Human, Mouse and Rat samples.
This antibody is suitable for WB, IHC, IF and ELISA applications. It has been validated by WB analysis of Neuro-2a. The target is localized to the cell membrane, multi-pass membrane protein, basolateral cell membrane, multi-pass membrane protein, Associates with CLDN1 and the CLDN1-CD81 complex localizes to the basolateral cell membrane.
Product Name:
Recombinant CD81 Monoclonal Antibody
SKU:
AGEL6050
Size:
50 µL, 100 µL
Antibody Type:
Recombinant Monoclonal Antibody
Synonyms:
26 kDa cell surface protein TAPA, 26 kDa cell surface protein TAPA-1, APA, APA1, CD81, CD81 antigen, CD81 molecule, CVID, CVID6, S5.7, TAPA-1, TAPA1, Target of the antiproliferative antibody 1. Tetraspanin, Target of the antiproliferative antibody 1. Tetraspanin-28, Tspan-28, TSPAN28
Reactivity:
Human, Mouse, Rat
Immunogen:
Recombinant Human CD81 protein
Applications:
WBIHCIFELISA
Clonality:
Recombinant Monoclonal
Clone No:
C110742
Host:
Rabbit
Isotype:
Rabbit IgG
Target:
CD81
Conjugation:
Unconjugated
Observed MW:
22 kDa
Calculated MW:
26 kDa
Recommended Dilution:
IHC 1:200-1000; WB 1:2000-10000; IF 1:200-1000; ELISA 1:5000-20000
UniProt:
P60033
Purification:
Protein A
Concentration:
0.2 mg/mL
Buffer:
PBS, 50% glycerol, 0.05% Proclin 300, 0.05% protein protectant.
Storage:
Store at -20°C valid for 12 months. Avoid freeze / thaw cycles.
Western Blot with Recombinant CD81 Monoclonal Antibody at dilution of 1:1000 dilution. Lane A: Neuro-2a lysate. Observed-MW:22 kDa Calculated-MW:26 kDa