The Recombinant CD86 Monoclonal Antibody is a recombinant rabbit monoclonal antibody (clone C511004) against CD86. This gene encodes a type I membrane protein that is a member of the immunoglobulin superfamily. This protein is expressed by antigen-presenting cells, and it is the ligand for two proteins at the cell surface of T cells, CD28 antigen and cytotoxic T-lymphocyte-associated protein 4. Binding of this protein with CD28 antigen is a costimulatory signal for activation of the T-cell. Produced by recombinant technology in Rabbit hosts and purified by Protein A affinity chromatography, this Rabbit IgG antibody is reactive against Mouse samples.
This antibody is suitable for WB, IHC, IF and ELISA applications. It has been validated by WB analysis of J774A.1. The target is localized to the membranous.
IHC 1:200-1000; WB 1:500-5000; IF 1:200-1000; ELISA 1:5000-20000
UniProt:
P42082
Purification:
Protein A
Concentration:
0.2 mg/mL
Buffer:
PBS, 50% glycerol, 0.05% Proclin 300, 0.05% protein protectant.
Storage:
Store at -20°C valid for 12 months. Avoid freeze / thaw cycles.
Western Blot with Recombinant CD86 Monoclonal Antibody at dilution of 1:1000 dilution. Lane A: J774A.1 whole cell lysate. Observed-MW:60-80 kDa Calculated-MW:60-80 kDa
