The Recombinant Cleaved Caspase-3 (Asp175) Monoclonal Antibody is a recombinant rabbit monoclonal antibody (clone C110982) against Cleaved Caspase-3. This gene encodes a protein which is a member of the cysteine-aspartic acid protease (caspase) family. Sequential activation of caspases plays a central role in the execution-phase of cell apoptosis. Caspases exist as inactive proenzymes which undergo proteolytic processing at conserved aspartic residues to produce two subunits, large and small, that dimerize to form the active enzyme. Produced by recombinant technology in Rabbit hosts and purified by Protein A affinity chromatography, this Rabbit IgG antibody is reactive against Human, Mouse and Rat samples.
This antibody is suitable for WB, IHC, IF, IP and ELISA applications. It has been validated by WB analysis of NIH-3T3 and IHC analysis of Human colon carcinoma. The target is localized to the cytoplasm.
Recombinant Human Cleaved Caspase-3 (Asp175) protein
Applications:
WBIHCIFIPELISA
Clonality:
Recombinant Monoclonal
Clone No:
C110982
Host:
Rabbit
Isotype:
Rabbit IgG
Target:
Cleaved Caspase-3
Conjugation:
Unconjugated
Observed MW:
17 kDa, 19 kDa
Calculated MW:
17 kDa, 19 kDa
Recommended Dilution:
IHC 1:200-1000; WB 1:1000-5000; IF 1:200-1000; ELISA 1:5000-20000; IP 1:50-200
UniProt:
P42574
Purification:
Protein A
Concentration:
0.2 mg/mL
Buffer:
PBS, 50% glycerol, 0.05% Proclin 300, 0.05% protein protectant.
Storage:
Store at -20°C valid for 12 months. Avoid freeze / thaw cycles.
Immunohistochemistry of paraffin-embedded human colon carcinoma using Recombinant Cleaved Caspase-3 (Asp175) Monoclonal Antibody at dilution of 1:200.
Western Blot with Recombinant Cleaved Caspase-3 (Asp175) Monoclonal Antibody at dilution of 1:1000 dilution. Lane A: NIH-3T3 cell lysate. Observed-MW:17 kDa,19 kDa Calculated-MW:17 kDa,19 kDa
