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Recombinant Creatine kinase B type Monoclonal Antibody (AGEL6889)

Datasheet MSDS
SKU AGEL6889
Product Type Antibody
Reactivity Human, Rat, Mouse
Applications WB, IHC-P, ICC/IF, FC
Host Species Rabbit
Clonality Monoclonal
Clone C111584
Conjugate Unconjugated
$606 - $843

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Description

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Recombinant Creatine kinase B type Monoclonal Antibody

The Recombinant Creatine kinase B type Monoclonal Antibody is a recombinant rabbit monoclonal antibody (clone C111584) against Creatine kinase B type. Creatine Kinase, an enzyme important for energy metabolism in cells of high and fluctuating energy requirements, catalyses the reversible transfer of a phosphoryl goup from phosphocreatine to ADP. CK isoenzymes play a central role in energy transduction in tissues with large, fluctuating energy demands, such as skeletal muscle, heart, brain and spermatozoa. Inactivation of creatine kinase by gliotoxin was accompanied by the formation of a 37 kDa form of the enzyme. This oxidized form of creatine kinase was rapidly reconverted to the 42-kDa species by the addition of reducing agents concomitant with restoration of activity. Produced by recombinant technology in Rabbit hosts and purified by Protein A affinity chromatography, this Rabbit IgG antibody is reactive against Human, Mouse and Rat samples.

This antibody is suitable for WB, IHC-P, ICC/IF and FC applications. It has been validated by WB analysis of K562, C6, HeLa, Rat brain, Mouse brain, IHC analysis of Human breast and IF analysis of HeLa. The target is localized to the cytoplasm, mitochondrion.

Product Name:Recombinant Creatine kinase B type Monoclonal Antibody
SKU:AGEL6889
Size:50 µL, 100 µL
Antibody Type:Recombinant Monoclonal Antibody
Synonyms:B-CK, BCK, CKB, CKBB, Creatine Kinase BB, HEL, HEL-211, HEL-S, HEL-S-29
Reactivity:Human, Mouse, Rat
Immunogen:A synthetic peptide of human Creatine kinase B type
Applications:WBIHC-PICC/IFFC
Clonality:Recombinant Monoclonal
Clone No:C111584
Host:Rabbit
Isotype:Rabbit IgG
Target:Creatine kinase B type
Conjugation:Unconjugated
Observed MW:43 kDa
Calculated MW:43 kDa
Recommended Dilution:WB 1:2000-20000; IHC-P 1:100-1000; ICC/IF 1:50-500; FCM 1:10-100
UniProt:P12277
Purification:Protein A
Concentration:1 mg/mL
Buffer:PBS, 50% glycerol, 0.05% Proclin 300, 0.05% protein protectant.
Storage:Store at -20°C valid for 12 months. Avoid freeze / thaw cycles.
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