Lymphocyte Activating 3(LAG3) belongs to immunoglobulin (Ig) superfamily, Macaca fascicularis LAG3 cDNA encodes 533 amino acids (aa) that include a 17 aa signal sequence, a 451 aa extracellular domain (ECD) with two Ig-like domains, a transmembrane region and a highly charged cytoplasmic region. LAG3's main ligand is MHC class II, to which it binds with higher affinity than CD4. The protein negatively regulates cellular proliferation, activation, and homeostasis of T cells, in a similar fashion to CTLA-4 and PD-1 and has been reported to play a role in Treg suppressive function.
Product Name:
Cynomolgus LAG-3/CD223 Recombinant Protein (RPES2511)
Product Code:
RPES2511
Size:
10µg
Species:
Cynomolgus
Expressed Host:
Human Cells
Synonyms:
Lymphocyte Activating 3, LAG3, LAG-3, CD223
Accession:
XP_005570011.1
Sequence:
Pro23-Leu450(Pro74)
Fusion tag:
N-His
Activity:
Immobilized Cynomolgus LAG-3-His at 2µg/ml(100 ?l/well) can bind Human FGL1-Fc. The ED50 of Cynomolgus LAG-3-His is 5.8 ug/ml .
Endotoxin:
<1.0 EU per µg as determined by the LAL method.
Protein Construction:
Recombinant Cynomolgus LAG-3 is produced by our Mammalian expression system and the target gene encoding Pro23-Leu450(Pro74) is expressed with a 8His tag at the N-terminus.
Purity:
> 95% as determined by reducing SDS-PAGE.
Mol Mass:
47.3 kDa
AP Mol Mass:
55-65&140 kDa
Formulation:
Lyophilized from a 0.2 µm filtered solution of PBS,PH7.4.
Shipping:
This product is provided as lyophilized powder which is shipped with ice packs.
Stability and Storage:
Lyophilized proteins are stable for up to 12 months when stored at -20 to -80°C. Reconstituted protein solution can be stored at 4-8°C for 2-7 days. Aliquots of reconstituted samples are stable at < -20°C for 3 months.
