The Recombinant E-Cadherin/CDH1/E-cad/CD324 Monoclonal Antibody is a recombinant rabbit monoclonal antibody (clone C310021) against CDH1. This gene encodes a classical cadherin of the cadherin superfamily. Alternative splicing results in multiple transcript variants, at least one of which encodes a preproprotein that is proteolytically processed to generate the mature glycoprotein. This calcium-dependent cell-cell adhesion protein is comprised of five extracellular cadherin repeats, a transmembrane region and a highly conserved cytoplasmic tail. Produced by recombinant technology in Rabbit hosts and purified by Protein A affinity chromatography, this Rabbit IgG antibody is reactive against Human samples.
This antibody is suitable for WB, IHC, ICC/IF and FC applications. It has been validated by WB analysis of A431, MCF-7, HepG2, Caco-2, IHC analysis of Human liver, Human breast cancer and IF analysis of MCF-7. The target is localized to the cell membrane, endosome, Golgi apparatus, cytoplasm.
Store at -20°C valid for 12 months. Avoid freeze / thaw cycles.
Immunofluorescent analysis of MCF-7 cells (green), and DAPI (blue).
Immunohistochemical analysis of paraffin-embedded Human breast cancer tissue sections using E- Cadherin/CDH1/E-cad/CD324 Antibody (RMEA0035) at a dilution of 1:300 (10× lens). Heat mediated antigen retrieval was performed with Tris-EDTA buffer (pH 9.0) for 20 min prior to IHC staining.
Immunohistochemical analysis of paraffin-embedded Human liver tissue sections using E-Cadherin/CDH1/E- cad/CD324 Antibody (RMEA0035) at a dilution of 1:300 (10× lens). Heat mediated antigen retrieval was performed with Tris-EDTA buffer (pH 9.0) for 20 min prior to IHC staining.
Western blot analysis of A431,MCF-7,HepG2 and Caco-2 cell lysates using E-Cadherin/CDH1/E-cad/CD324 Antibody (RMEA0035) at a dilution of 1:1500. Observed-MW:80-120kDa,135 kDa Calculated-MW:98 kDa
