The Recombinant Ezrin Monoclonal Antibody is a recombinant rabbit monoclonal antibody (clone C110505) against Ezrin. The cytoplasmic peripheral membrane protein encoded by this gene functions as a protein-tyrosine kinase substrate in microvilli. As a member of the ERM protein family, this protein serves as an intermediate between the plasma membrane and the actin cytoskeleton. This protein plays a key role in cell surface structure adhesion, migration and organization, and it has been implicated in various human cancers. Produced by recombinant technology in Rabbit hosts and purified by Protein A affinity chromatography, this Rabbit IgG antibody is reactive against Human, Mouse and Rat samples.
This antibody is suitable for WB, IHC, IF, IP and ELISA applications. It has been validated by WB analysis of HCT-116. The target is localized to the cytoplasm, membrane.
Product Name:
Recombinant Ezrin Monoclonal Antibody
SKU:
AGEL5820
Size:
50 µL, 100 µL
Antibody Type:
Recombinant Monoclonal Antibody
Synonyms:
CVIL, CVL, Cytovillin, Cytovillin 2, DFNB, DFNB24, DKFZp762H, DKFZp762H157, Epididymis secretory protein Li, Epididymis secretory protein Li 105, EZR, EZRI, Ezrin, FLJ26216, HEL, HEL S, HEL S 105, HEL-S, HEL-S-105, HEL70, IMD, IMD50, MGC, MGC1584, p81, VIL, VIL 2, VIL2, Villin 2, Villin 2 (ezrin), Villin 2 ezrin, Villin-2, Villin2
Reactivity:
Human, Mouse, Rat
Immunogen:
Recombinant Human Ezrin protein
Applications:
WBIHCIFIPELISA
Clonality:
Recombinant Monoclonal
Clone No:
C110505
Host:
Rabbit
Isotype:
Rabbit IgG
Target:
Ezrin
Conjugation:
Unconjugated
Observed MW:
80 kDa
Calculated MW:
69 kDa
Recommended Dilution:
IHC 1:1000-4000; WB 1:2000-10000; IF 1:200-1000; ELISA 1:5000-20000; IP 1:50-200
UniProt:
P15311
Purification:
Protein A
Concentration:
0.2 mg/mL
Buffer:
PBS, 50% glycerol, 0.05% Proclin 300, 0.05% protein protectant.
Storage:
Store at -20°C valid for 12 months. Avoid freeze / thaw cycles.
Western Blot with Recombinant Ezrin Monoclonal Antibody at dilution of 1:1000 dilution. Lane A: HCT-116 cells. Observed-MW:80 kDa Calculated-MW:69 kDa