The Recombinant GLP-1 Monoclonal Antibody is a recombinant rabbit monoclonal antibody (clone C111153) against GLP-1. Glucagon plays a key role in glucose metabolism and homeostasis. Regulates blood glucose by increasing gluconeogenesis and decreasing glycolysis. A counterregulatory hormone of insulin, raises plasma glucose levels in response to insulin-induced hypoglycemia. Produced by recombinant technology in Rabbit hosts and purified by Protein A affinity chromatography, this Rabbit IgG antibody is reactive against Human, Mouse and Rat samples.
This antibody is suitable for WB, IHC, IF and FCM applications. It has been validated by WB analysis of Rat pancreas, Mouse pancreas, IHC analysis of Human pancreas, Mouse pancreas, Rat pancreas, IF analysis of PANC-1 and FCM analysis of PANC-1. The target is localized to the secreted.
WB 1:500-2000; IHC 1:200-1000; IF 1:50; FCM 1:50-100
UniProt:
P01275
Purification:
Protein A
Concentration:
1 mg/mL
Buffer:
PBS, 50% glycerol, 0.05% Proclin 300, 0.05% protein protectant.
Storage:
Store at -20°C valid for 12 months. Avoid freeze / thaw cycles.
Flow cytometric analysis of human GLP-1 expression on PANC-1 cells. Cells were stained with purified anti-Human GLP-1, then a Alexa Fluor 488-conjugated second step antibody. The histogram were derived from events with the forward and side light-scatter characteristics of intact cells.
Immunofluorescent analysis of (4% Paraformaldehyde) fixed PANC-1 cells using anti-GLP-1 Monoclonal Antibody at dilution of 1:50.
Immunohistochemistry of paraffin-embedded Human pancreas using GLP-1 Monoclonal Antibody at dilution of 1: 1000.
Immunohistochemistry of paraffin-embedded Mouse pancreas using GLP-1 Monoclonal Antibody at dilution of 1:1000.
Immunohistochemistry of paraffin-embedded Rat pancreas using GLP-1 Monoclonal Antibody at dilution of 1:1000.
Western Blot with GLP-1 Monoclonal Antibody at dilution of 1:2000. Lane 1: Rat pancreas, Lane 2: Mouse pancreas Observed-MW:11 kDa Calculated-MW:21 kDa
