The Recombinant Glucocorticoid Receptor Monoclonal Antibody is a recombinant rabbit monoclonal antibody (clone C110626) against Glucocorticoid Receptor. This gene encodes glucocorticoid receptor, which can function both as a transcription factor that binds to glucocorticoid response elements in the promoters of glucocorticoid responsive genes to activate their transcription, and as a regulator of other transcription factors. This receptor is typically found in the cytoplasm, but upon ligand binding, is transported into the nucleus. It is involved in inflammatory responses, cellular proliferation, and differentiation in target tissues. Produced by recombinant technology in Rabbit hosts and purified by Protein A affinity chromatography, this Rabbit IgG antibody is reactive against Human, Mouse and Rat samples.
This antibody is suitable for WB, IHC, IF and ELISA applications. It has been validated by WB analysis of U-251 MG and IHC analysis of Human liver tissue, Rat liver tissue. The target is localized to the nucleus.
GCCR, GCR, GCRST, glucocorticoid nuclear receptor variant, glucocorticoid nuclear receptor variant 1, Glucocorticoid receptor, GR, GRL, Grl1, group C, member 1 (glucocorticoid receptor), NR3C, NR3C1, nuclear receptor subfamily, nuclear receptor subfamily 3, Nuclear receptor subfamily 3 group C member, Nuclear receptor subfamily 3 group C member 1
Reactivity:
Human, Mouse, Rat
Immunogen:
Recombinant Human Glucocorticoid Receptor protein
Applications:
WBIHCIFELISA
Clonality:
Recombinant Monoclonal
Clone No:
C110626
Host:
Rabbit
Isotype:
Rabbit IgG
Target:
Glucocorticoid Receptor
Conjugation:
Unconjugated
Observed MW:
94 kDa
Calculated MW:
85 kDa
Recommended Dilution:
IHC 1:1000-4000; WB 1:1000-5000; IF 1:200-1000; ELISA 1:5000-20000
UniProt:
P04150
Purification:
Protein A
Concentration:
0.2 mg/mL
Buffer:
PBS, 50% glycerol, 0.05% Proclin 300, 0.05% protein protectant.
Storage:
Store at -20°C valid for 12 months. Avoid freeze / thaw cycles.
Immunohistochemistry of paraffin-embedded human liver tissue using Recombinant Glucocorticoid Receptor Monoclonal Antibody at dilution of 1:200.
Immunohistochemistry of paraffin-embedded rat liver tissue using Recombinant Glucocorticoid Receptor Monoclonal Antibody at dilution of 1:200.
Western Blot with Recombinant Glucocorticoid Receptor Monoclonal Antibody at dilution of 1:1000 dilution. Lane A: U-251 MG cells. Observed-MW:94 kDa Calculated-MW:85 kDa