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Recombinant GRP94 Monoclonal Antibody (RMEA1166)

Datasheet MSDS
SKU RMEA1166
Product Type Antibody
Reactivity Human, Rat, Mouse
Applications WB, IF, IHC, IP
Host Species Rabbit
Clonality Monoclonal
Clone C111165
Conjugate Unconjugated
$606 - $843

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Description

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Recombinant GRP94 Monoclonal Antibody

The Recombinant GRP94 Monoclonal Antibody is a recombinant rabbit monoclonal antibody (clone C111165) against GRP94. Molecular chaperone that functions in the processing and transport of secreted proteins. When associated with CNPY3, required for proper folding of Toll-like receptors. Functions in endoplasmic reticulum associated degradation (ERAD). Produced by recombinant technology in Rabbit hosts and purified by Protein A affinity chromatography, this Rabbit IgG antibody is reactive against Human, Mouse and Rat samples.

This antibody is suitable for WB, IHC, IF and IP applications. It has been validated by WB analysis of HeLa, MCF-7, MOLT-4, Rat brian, Mouse brain, IF analysis of MCF7, IHC analysis of Human colon cancer, Human lung squamous carcinoma and IP analysis of HeLa cells extracts. The target is localized to the endoplasmic reticulum.

Product Name:Recombinant GRP94 Monoclonal Antibody
SKU:RMEA1166
Size:50 µL, 100 µL
Antibody Type:Recombinant Monoclonal Antibody
Synonyms:90 kDa, 94 kDa glucose regulated protein, 94 kDa glucose-regulated protein, beta, ECGP, endoplasmin, Endothelial cell (HBMEC) glycoprotein, ENPL, ERp99, Glucose regulated protein 94kDa, GP96, gp96 homolog, GRP, GRP 94, GRP-94, GRP94, Heat shock protein, Heat shock protein 90 kDa beta member 1, heat shock protein 90kDa beta (Grp94), Heat shock protein 90kDa beta member 1, HEL, HEL-S-125m, HEL35, HSP90B, HSP90B1, member 1, Stress inducible tumor rejection antigen GP96, TRA, TRA1, tumor rejection antigen (gp96) 1, Tumor rejection antigen 1, Tumor rejection antigen gp96, Tumor rejection antigen-1 (gp96)
Reactivity:Human, Mouse, Rat
Immunogen:Recombinant human GRP94 fragment
Applications:WBIHCIFIP
Clonality:Recombinant Monoclonal
Clone No:C111165
Host:Rabbit
Isotype:Rabbit IgG
Target:GRP94
Conjugation:Unconjugated
Observed MW:100 kDa
Calculated MW:94 kDa
Recommended Dilution:WB 1:500-2000; IHC 1:200-1000; IF 1:50; IP 1:25-100
UniProt:P14625
Purification:Protein A
Concentration:1 mg/mL
Buffer:PBS, 50% glycerol, 0.05% Proclin 300, 0.05% protein protectant.
Storage:Store at -20°C valid for 12 months. Avoid freeze / thaw cycles.
Product ImageImmunofluorescent analysis of (100% methanol) fixed MCF7 cells using anti-GRP94 Monoclonal Antibody at dilution of 1:50.
Product ImageImmunohistochemistry of paraffin-embedded Human colon cancer using GRP94 Monoclonal Antibody at dilution of 1:1000.
Product ImageImmunohistochemistry of paraffin-embedded Human lung squamous carcinoma using GRP94 Monoclonal Antibody at dilution of 1:50.
Product ImageImmunoprecipitation analysis using anti-GRP94 Monoclonal Antibody. Western blot was performed from the immunoprecipitate using GRP94 Monoclonal Antibody at a dilution of 1:100. Lane 1: 5% Input, Lane 2: GRP94 Monoclonal Antibody, Lane 3: Rabbit monoclonal IgG Isotype Observed-MW:100 kDa Calculated-MW:94 kDa
Product ImageWestern Blot with GRP94 Monoclonal Antibody at dilution of 1:2000. Lane 1: HeLa, Lane 2: MCF-7, Lane 3: MOLT-4, Lane 4: Rat brian, Lane 5: Mouse brain Observed-MW:100 kDa Calculated-MW:94 kDa
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