The Recombinant Histone H3 Monoclonal Antibody is a recombinant rabbit monoclonal antibody (clone C110480) against Histone H3. Histones are basic nuclear proteins that are responsible for the nucleosome structure of the chromosomal fiber in eukaryotes. This structure consists of approximately 146 bp of DNA wrapped around a nucleosome, an octamer composed of pairs of each of the four core histones (H2A, H2B, H3, and H4). The chromatin fiber is further compacted through the interaction of a linker histone, H1, with the DNA between the nucleosomes to form higher order chromatin structures. Produced by recombinant technology in Rabbit hosts and purified by Protein A affinity chromatography, this Rabbit IgG antibody is reactive against Human, Mouse and Rat samples.
This antibody is suitable for WB, IHC, IF, IP, ELISA, CHIP and Cut&Tag applications. It has been validated by WB analysis of HeLa, HepG2, A431, Jurkat, 293T, RAW 264.7, NIH/3T3, Mouse kindey, Mouse brain, Mouse liver, Rat kindey, Rat brain, Rat liver. The target is localized to the nucleus.
IHC 1:5000-20000; WB 1:5000-20000; IF 1:200-1000; ELISA 1:5000-20000; IP 1:50-200; CHIP 1:50-100; Cut&Tag 1:50-100
UniProt:
P68431
Purification:
Protein A
Concentration:
0.2 mg/mL
Buffer:
PBS, 50% glycerol, 0.05% Proclin 300, 0.05% protein protectant.
Storage:
Store at -20°C valid for 12 months. Avoid freeze / thaw cycles.
Western blot analysis of HeLa,HepG2,A431,Jurkat, 293T,RAW 264.7,NIH/3T3,Mouse kindey,Mouse brain,Mouse liver,Rat kindey,Rat brain and Rat liver cell lysates using Histone H3 Antibody (RMEA0491) at a dilution of 1:7500. Observed-MW:15 kDa Calculated-MW:15 kDa
