Envelope glycoprotein gp160 is single-pass type I membrane protein. The mature envelope protein (Env) consists of a homotrimer of non-covalently associated gp120-gp41 heterodimers. It is cleaved into the following 2 chains: glycoprotein 120 and transmembrane protein gp41. The resulting complex protrudes from the virus surface as a spike. The 17 amino acids long immunosuppressive region is present in many retroviral envelope proteins. Synthetic peptides derived from this relatively conserved sequence inhibit immune function in vitro and in vivo.
Product Name:
HIV HIV gp120 Recombinant Protein (RPES3514)
Product Code:
RPES3514
Size:
10µg
Species:
HIV
Expressed Host:
Human Cells
Synonyms:
Envelope glycoprotein gp120, Glycoprotein 120,Surface protein gp120
Accession:
Q9DKG6
Sequence:
Met1-Glu498
Fusion tag:
C-His
Endotoxin:
<1.0 EU per µg as determined by the LAL method.
Protein Construction:
Recombinant HIV 1 Envelope glycoprotein gp120 is produced by our Mammalian expression system and the target gene encoding Met1-Glu498 is expressed with a 8His tag at the C-terminus.
Purity:
> 90% as determined by reducing SDS-PAGE.
Mol Mass:
57 kDa
AP Mol Mass:
105-130 kDa
Formulation:
Lyophilized from a 0.2 µm filtered solution of PBS, pH7.4.
Shipping:
This product is provided as lyophilized powder which is shipped with ice packs.
Stability and Storage:
Lyophilized proteins are stable for up to 12 months when stored at -20 to -80°C. Reconstituted protein solution can be stored at 4-8°C for 2-7 days. Aliquots of reconstituted samples are stable at < -20°C for 3 months.
