The Recombinant HLA-DRA Monoclonal Antibody is a recombinant rabbit monoclonal antibody (clone C351175) against HLA-DRA. An alpha chain of antigen-presenting major histocompatibility complex class II (MHCII) molecule. In complex with the beta chain HLA-DRB, displays antigenic peptides on professional antigen presenting cells (APCs) for recognition by alpha-beta T cell receptor (TCR) on HLA-DR-restricted CD4-positive T cells. Produced by recombinant technology in Rabbit hosts and purified by Protein A affinity chromatography, this Rabbit IgG antibody is reactive against Human samples.
This antibody is suitable for IHC and FCM applications. It has been validated by IHC analysis of Human liver, Human spleen and FCM analysis of PBMC. The target is localized to the membrane.
Product Name:
Recombinant HLA-DRA Monoclonal Antibody
SKU:
AGEL6481
Size:
50 µL, 100 µL
Antibody Type:
Recombinant Monoclonal Antibody
Synonyms:
class II, DR alpha, DR alpha chain, DR alpha chain precursor, DRA, DRB1, DRB4, Histocompatibility antigen HLA DR alpha, HLA class II histocompatibility antigen, HLA class II histocompatibility antigen DR alpha chain, HLA DR1B, HLA DR3B, HLA DRA, HLA DRA1, HLA DRB1, HLA DRB3, HLA DRB4, HLA DRB5, HLA-DRA, HLA-DRA1, HLADR4B, HLADRA1, HLADRB, major histocompatibility complex, Major histocompatibility complex class II DR alpha, Major histocompatibility complex class II DR beta 1, Major histocompatibility complex class II DR beta 3, Major histocompatibility complex class II DR beta 4, Major histocompatibility complex class II DR beta 5, MGC117330, MHC cell surface glycoprotein, MHC class II antigen DRA, MHC II, MLRW
Reactivity:
Human
Immunogen:
Recombinant human HLA-DRA fragment
Applications:
IHCFCM
Clonality:
Recombinant Monoclonal
Clone No:
C351175
Host:
Rabbit
Isotype:
Rabbit IgG
Target:
HLA-DRA
Conjugation:
Unconjugated
Recommended Dilution:
IHC 1:50-100; FCM 1:50
UniProt:
P01903
Purification:
Protein A
Concentration:
1 mg/mL
Buffer:
PBS, 50% glycerol, 0.05% Proclin 300, 0.05% protein protectant.
Storage:
Store at -20°C valid for 12 months. Avoid freeze / thaw cycles.
Flow cytometric analysis of human HLA-DRA expression on PBMC cells. Cells were stained with purified anti- Human HLA-DRA, then a Alexa Fluor 488-conjugated second step antibody. The histogram were derived from events with the forward and side light-scatter characteristics of intact cells.
Immunohistochemistry of paraffin-embedded Human liver using HLA-DRA Monoclonal Antibody at dilution of 1:100.
Immunohistochemistry of paraffin-embedded Human spleen using HLA-DRA Monoclonal Antibody at dilution of 1:100.
