Recombinant Human ADH7 Protein (His Tag) (RPES2207)

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SKU:
RPES2207
€196

Description

ELISA Kit Technical Manual

Recombinant Human ADH7 Protein (His Tag)

Background:Alcohol dehydrogenase class 4 mu/sigma chain (ADH7) is a cytoplasm enzyme which is a member of the alcohol dehydrogenase family. The expression of this gene makes it much more abundant in the stomach than the liver, thus it differs from the other known gene family members. ADH7 may participate in the synthesis of retinoic acid, a hormone important for cellular differentiation. Medium-chain (octanol) and aromatic (m-nitrobenzaldehyde) compounds are the best substrates. Ethanol is not a good substrate but at the high ethanol concentrations reached in the digestive tract, it plays a role in the ethanol oxidation and contributes to the first pass ethanol metabolism.
Name:Recombinant Human ADH7 Protein (His Tag)
Size:10µg
Assay Genie SKU:RPES2207
Synonyms:Alcohol Dehydrogenase Class 4 Mu/Sigma Chain; Alcohol Dehydrogenase Class IV Mu/Sigma Chain; Gastric Alcohol Dehydrogenase; Retinol Dehydrogenase; ADH7
Species:Human
Expression host:Human Cells
Sequence:Met 1-Phe386
Accession:P40394
Mol Mass:42.5 kDa
AP Mol Mass:44 kDa
Tag:C-6His
Purity:> 95 % as determined by reducing SDS-PAGE.
Endotoxin:< 1.0 EU per µg as determined by the LAL method.
Storage:Lyophilized proteins are stable for up to 12 months when stored at -20 to -80°C. Reconstituted protein solution can be stored at 4-8? for 2-7 days. Aliquots of reconstituted samples are stable at < -20'C for 3 months.
Shipping:This product is provided as lyophilized powder which is shipped with ice packs.
Formulation:Lyophilized from a 0.2 um filtered solution of PBS, pH7.4.
Reconstitution:Please refer to the printed manual for detailed information.
UniProt Protein Function:ADH7: Could function in retinol oxidation for the synthesis of retinoic acid, a hormone important for cellular differentiation. Medium-chain (octanol) and aromatic (m-nitrobenzaldehyde) compounds are the best substrates. Ethanol is not a good substrate but at the high ethanol concentrations reached in the digestive tract, it plays a role in the ethanol oxidation and contributes to the first pass ethanol metabolism. Belongs to the zinc-containing alcohol dehydrogenase family. Class-IV subfamily. 2 isoforms of the human protein are produced by alternative splicing.
UniProt Protein Details:

Protein type:Xenobiotic Metabolism - drug metabolism - cytochrome P450; Xenobiotic Metabolism - metabolism by cytochrome P450; Oxidoreductase; Cofactor and Vitamin Metabolism - retinol; Carbohydrate Metabolism - glycolysis and gluconeogenesis; Amino Acid Metabolism - tyrosine; EC 1.1.1.1; Lipid Metabolism - fatty acid

Chromosomal Location of Human Ortholog: 4q23-q24

Cellular Component: cytosol

Molecular Function:alcohol dehydrogenase activity; alcohol dehydrogenase activity, zinc-dependent; aldehyde oxidase activity; ethanol binding; receptor antagonist activity; retinol binding; retinol dehydrogenase activity

Biological Process: ethanol oxidation; fatty acid omega-oxidation; response to bacterium; response to ethanol; retinoid metabolic process

NCBI Summary:This gene encodes class IV alcohol dehydrogenase 7 mu or sigma subunit, which is a member of the alcohol dehydrogenase family. Members of this family metabolize a wide variety of substrates, including ethanol, retinol, other aliphatic alcohols, hydroxysteroids, and lipid peroxidation products. The enzyme encoded by this gene is inefficient in ethanol oxidation, but is the most active as a retinol dehydrogenase; thus it may participate in the synthesis of retinoic acid, a hormone important for cellular differentiation. The expression of this gene is much more abundant in stomach than liver, thus differing from the other known gene family members. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Oct 2009]
UniProt Code:P40394
NCBI GenInfo Identifier:292495000
NCBI Gene ID:131
NCBI Accession:P40394.2
UniProt Secondary Accession:P40394,Q13713, A2RRB6, A8MVN9, B2R760, B4DWV6,
UniProt Related Accession:P40394
Molecular Weight:42,467 Da
NCBI Full Name:Alcohol dehydrogenase class 4 mu/sigma chain
NCBI Synonym Full Names:alcohol dehydrogenase 7 (class IV), mu or sigma polypeptide
NCBI Official Symbol:ADH7  
NCBI Official Synonym Symbols:ADH4  
NCBI Protein Information:alcohol dehydrogenase class 4 mu/sigma chain
UniProt Protein Name:Alcohol dehydrogenase class 4 mu/sigma chain
UniProt Synonym Protein Names:Alcohol dehydrogenase class IV mu/sigma chain; Gastric alcohol dehydrogenase; Retinol dehydrogenase
Protein Family:Alcohol dehydrogenase
UniProt Gene Name:ADH7  
UniProt Entry Name:ADH7_HUMAN
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Additional Information

Product type:
Recombinant Protein
Host species:
Human Cells
Reactivity:
Human
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