Human ASF1A Recombinant Protein (RPES0825)
- SKU:
- RPES0825
- Product Type:
- Recombinant Protein
- Species:
- Human
- Research Area:
- Epigenetics & Nuclear Signaling
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Description
system_update_altDatasheet
Human ASF1A Recombinant Protein
Human Histone Chaperone ASF1A (ASF1A) belongs to the H3/H4 family of histone chaperone proteins. ASF1A is ubiquitously expressed in many cells and tissues, interacting with histones H3 and H4. ASF1A cooperates with Chromatin Assembly Factor 1 to promote replication-dependent chromatin assembly and with HIRA to promote replication-independent chromatin assembly. In addition, ASF1A is necessary for the formation of senescence-associated heterochromatin foci (SAHF) and efficient senescence-associated cell cycle exit.
| Product Name: | Human ASF1A Recombinant Protein (RPES0825) |
| Product Code: | RPES0825 |
| Size: | 10µg |
| Species: | Human |
| Expressed Host: | E.coli |
| Synonyms: | Histone Chaperone ASF1A, Anti-Silencing Function Protein 1 Homolog A, hAsf1, hAsf1a, CCG1-Interacting Factor A, CIA, hCIA, ASF1A |
| Accession: | Q9Y294 |
| Sequence: | Met 1-Met204 |
| Fusion tag: | C-6His |
| Endotoxin: | <1.0 EU per µg as determined by the LAL method. |
| Protein Construction: | Recombinant Human Histone Chaperone ASF1A is produced by our E.coli expression system and the target gene encoding Met1-Met204 is expressed with a T7 tag at the N-terminus, 6His tag at the C-terminus. |
| Purity: | > 95 % as determined by reducing SDS-PAGE. |
| Mol Mass: | 25.4 kDa |
| AP Mol Mass: | 22 kDa |
| Formulation: | Lyophilized from a 0.2 µm filtered solution of 20mM TrisHCl, 1mM DTT, 150mM NaCl, pH 8.0. |
| Shipping: | This product is provided as lyophilized powder which is shipped with ice packs. |
| Stability and Storage: | Lyophilized proteins are stable for up to 12 months when stored at -20 to -80°C. Reconstituted protein solution can be stored at 4-8°C for 2-7 days. Aliquots of reconstituted samples are stable at < -20°C for 3 months. |
