ATG3 (Ubiquitin-like-conjugating enzyme ATG3), also known as Apg3L and Apg3p, is a regulatory component of autophagosome biogenesis required for autophagy. ATG3 exhibits 98% aa sequence identity with both its mouse and rat orthologs. It is highly concentrated in the heart, skeletal muscle, kidneys, livers tissues, and placenta. As an E2-like enzyme, involves in autophagy and mitochondrial homeostasis. ATG8-like proteins are conjugated to PE by ATG3, which is required for autophagy. ATG3 can also act as a substrate for the conjugation of ATG12 to itself, which is required for mitochondrial homeostasis but not autophagy. Human ATG3 Recombinant Protein is a highly pure recombinant protein developed by Assay Genie for use in a range of applications.
Product Name:
Human ATG3 Recombinant Protein (RPES3124)
Product Code:
RPES3124
Size:
10µg
Species:
Human
Expressed Host:
E.coli
Synonyms:
Ubiquitin-like-conjugating enzyme ATG3, Autophagy-related protein 3, APG3-like, hApg3, Protein PC3-96, ATG3, APG3, APG3L
Accession:
Q9NT62
Sequence:
Met 1-Met314
Endotoxin:
<1.0 EU per µg as determined by the LAL method.
Protein Construction:
Recombinant Human Ubiquitin-like-conjugating Enzyme ATG3/ATG3 is produced by our E.coli expression system and the target gene encoding Met1-Met314 is expressed.
Purity:
> 95 % as determined by reducing SDS-PAGE.
Mol Mass:
36 kDa
AP Mol Mass:
38 kDa
Formulation:
Supplied as a 0.2 µm filtered solution of 20mM TrisHCl,150mM NaCl,10% Glycerol,pH 8.0.
Shipping:
This product is provided as liquid. It is shipped at frozen temperature with blue ice/gel packs.Upon receipt, store it immediately at<-20°C.
Stability and Storage:
Store at < -20°C, stable for 6 months. Please minimize freeze-thaw cycles.
