Recombinant Human Beta-2-Microglobulin/B2M Protein (His Tag) (RPES4170)

SKU:
RPES4170
€142

Description

ELISA Kit Technical Manual

Recombinant Human Beta-2-Microglobulin/B2M Protein (His Tag)

Background:?-2-Microglobulin (B2M) is a secreted protein with 1 Ig-like C1-type (immunoglobulin-like) domain which belongs to the beta-2-microglobulin family. B2M component of major histocompatibility complex (MHC) class I molecules, involved in the presentation of peptide antigens to the immune system. Polymers of beta 2-microglobulin can be found in tissues from patients on long-term hemodialysis. B2M is a protein found on the surface of many cells and plentiful on the surface of white blood cells. Serum B2M concentration is increased in renal diseases, various malignant diseases and some inflammatory and autoimmune disorders. B2M may adopt the fibrillar configuration of amyloid in certain pathologic states. The capacity to assemble into amyloid fibrils is concentration dependent. B2M has been shown as a marker for monitoring inflammatory disease activity and it appears likely to have a destructive role in amyloidosis-related arthritis. B2M might be involved in the OA (osteoarthritis) pathogenesis. Defects in B2M are the cause of hypercatabolic hypoproteinemia. Affected individuals show marked reduction in serum concentrations of immunoglobulin and albumin, probably due to rapid degradation. B2M could be a potential therapeutic target in ovarian cancer.
Name:Recombinant Human Beta-2-Microglobulin/B2M Protein (His Tag)
Size:10µg
Assay Genie SKU:RPES4170
Synonyms:Beta-2-Microglobulin; B2M; ?2-Microglobulin
Species:Human
Expression host:Human Cells
Sequence:Ile21-Met119
Accession:P61769
Mol Mass:12.77 kDa
AP Mol Mass:14 kDa
Tag:C-His
Purity:> 95% as determined by reducing SDS-PAGE.
Endotoxin:< 1.0 EU per ?g as determined by the LAL method.
Storage:Lyophilized protein should be stored at < -20'C, though stable at room temperature for 3 weeks.Reconstituted protein solution can be stored at 4-7? for 2-7 days.Aliquots of reconstituted samples are stable at < -20'C for 3 months.
Shipping:This product is provided as lyophilized powder which is shipped with ice packs.
Formulation:Lyophilized from a 0.2 um filtered solution of 20mM PB, 150mM NaCl, pH 7.2.
Reconstitution:Please refer to the printed manual for detailed information.
UniProt Protein Function:B2M: Component of the class I major histocompatibility complex (MHC). Involved in the presentation of peptide antigens to the immune system. Heterodimer of an alpha chain and a beta chain. Beta-2- microglobulin is the beta-chain of major histocompatibility complex class I molecules. Polymers of beta 2-microglobulin can be found in tissues from patients on long-term hemodialysis. Belongs to the beta-2-microglobulin family.
UniProt Protein Details:

Protein type:Secreted; Secreted, signal peptide

Chromosomal Location of Human Ortholog: 15q21.1

Cellular Component: Golgi membrane; Golgi apparatus; extracellular space; phagocytic vesicle membrane; focal adhesion; membrane; early endosome membrane; endoplasmic reticulum lumen; cytoplasm; plasma membrane; extracellular region; MHC class I protein complex; external side of plasma membrane

Molecular Function:identical protein binding; protein binding

Biological Process: response to drug; regulation of immune response; viral reproduction; positive regulation of T cell mediated cytotoxicity; antigen processing and presentation of exogenous peptide antigen via MHC class I, TAP-independent; cytokine and chemokine mediated signaling pathway; T cell differentiation in the thymus; antigen processing and presentation of exogenous peptide antigen via MHC class I, TAP-dependent; regulation of defense response to virus by virus; retinal homeostasis; iron ion homeostasis; antigen processing and presentation of peptide antigen via MHC class I; response to cadmium ion; response to molecule of bacterial origin; antigen processing and presentation of exogenous peptide antigen via MHC class I; innate immune response; protein refolding; antigen processing and presentation of exogenous protein antigen via MHC class Ib, TAP-dependent

Disease: Hypoproteinemia, Hypercatabolic

NCBI Summary:This gene encodes a serum protein found in association with the major histocompatibility complex (MHC) class I heavy chain on the surface of nearly all nucleated cells. The protein has a predominantly beta-pleated sheet structure that can form amyloid fibrils in some pathological conditions. The encoded antimicrobial protein displays antibacterial activity in amniotic fluid. A mutation in this gene has been shown to result in hypercatabolic hypoproteinemia.[provided by RefSeq, Aug 2014]
UniProt Code:P61769
NCBI GenInfo Identifier:48428791
NCBI Gene ID:567
NCBI Accession:P61769.1
UniProt Related Accession:P61769
Molecular Weight:
NCBI Full Name:Beta-2-microglobulin
NCBI Synonym Full Names:beta-2-microglobulin
NCBI Official Symbol:B2M  
NCBI Official Synonym Symbols:IMD43  
NCBI Protein Information:beta-2-microglobulin
UniProt Protein Name:Beta-2-microglobulin
Protein Family:Beta-2-microglobulin
UniProt Gene Name:B2M  
UniProt Entry Name:B2MG_HUMAN
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Additional Information

Product type:
Recombinant Protein
Host species:
Human Cells
Reactivity:
Human
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