CCL16 is a member of CC chemokine family. CCL16 cDNA encodes a 120 amino acid protein as well as a 23 amino acids signal peptide that is cleaved to give rise to 97 amino acid protein. CC chemokine CCL16 is distantly related to other CC chemokines, with less than 30% sequence identity. CCL16 elicits its effects on cells by interacting with cell surface chemokine receptors such as CCR1, CCR2, CCR5 and CCR8. Recombinant CCL16 has been demonstrated to attract human monocytes and THP1 cells, but not resting lymphocytes or neutrophils. CCL16 shows potent myelosuppressive activity and inhibits the proliferation of myeloid progenitor cells. CCL16 activates a calcium influx in THP1 cells that can be desensitized by prior RANTES treatment, suggesting that CCL16 and RANTES activate the same receptor in THP1 cells. Human CCL16 Recombinant Protein is a highly pure recombinant protein developed by Assay Genie for use in a range of applications.
Recombinant Human C-C Motif Chemokine 16 is produced by our E.coli expression system and the target gene encoding Gln24-Gln120 is expressed.
Purity:
> 95 % as determined by reducing SDS-PAGE.
Mol Mass:
11.0 kDa
AP Mol Mass:
14 kDa
Formulation:
Lyophilized from a 0.2 µm filtered solution of 20mM PB, 150mM NaCl, pH 7.4.
Shipping:
This product is provided as lyophilized powder which is shipped with ice packs.
Stability and Storage:
Lyophilized proteins are stable for up to 12 months when stored at -20 to -80°C. Reconstituted protein solution can be stored at 4-8°C for 2-7 days. Aliquots of reconstituted samples are stable at < -20°C for 3 months.
