Receptors for the Fc region of immunoglobin G (Fc?R) are divided into three classes and Fc?RIII is a multifunctional; low/intermediate affinity receptor. In humans; Fc?RIII is expressed as two distinct forms (Fc?RIIIA and Fc?RIIIB) that are encoded by two different but highly homologous genes in a cell type-specific manner. Fc?RIIIB is a low-affinity; GPI-linked receptor expressed by neutrophils and eosinophils; whereas Fc?RIIIA is an intermediate affinity polypeptide-anchored transmembrane glycoprotein expressed by a subset of T lymphocytes; natural killer (NK) cells; monocytes; and macrophages. The Fc?RIIIA receptor is involved in phagocytosis; secretion of enzymes; inflammatory mediators; antibody-dependent cellular cytotoxicity (ADCC); mast cell degranulation; and clearance of immune complexes. Fc?RIIIA has an immunoreceptor tyrosine-based activation motif (ITAM) in its cytoplasmic domain and delivers an activation signal in the immune responses. Aberrant expression or mutations in this gene is implicated in susceptibility to recurrent viral infections; systemic lupus erythematosus; and alloimmune neonatal neutropenia. In humans; it is a 50 -70 kD type I transmembrane activating receptor. The Fc?RIIIA cDNA encodes 254 amino acid including a 16aa signal sequence; 191 amino acid ECD with two C2-type Ig-like domains; five potential N-glycosylation sites; a 22 amino acid transmembrane sequence and a 25 amino acid cytoplasmic domain.
Product Name:
Human CD16a/FCGR3A Recombinant Protein (RPES3567)
Product Code:
RPES3567
Size:
10µg
Species:
Human
Expressed Host:
Human Cells
Synonyms:
Low Affinity Immunoglobulin Gamma Fc Region Receptor III-A, CD16a Antigen, Fc-Gamma RIII-Alpha, Fc-Gamma RIII, Fc-gamma RIIIa, FcRIII, FcRIIIa, FcR-10, IgG Fc Receptor III-2, CD16a, FCGR3A, CD16A, FCG3, FCGR3, IGFR3,CD16,CD16A
Accession:
P08637
Sequence:
Gly17-Gln208
Fusion tag:
C-6His
Activity:
Immobilized Human IgG1Fc(Cat: PKSH032558) at 10µg/ml(100 ?l/well) can bind Human CD16a-His. The ED50 of Human CD16a-His is 2.57 ug/ml .
Endotoxin:
<1.0 EU per µg as determined by the LAL method.
Protein Construction:
Recombinant Human Low Affinity Immunoglobulin Gamma Fc Region Receptor III-A is produced by our Mammalian expression system and the target gene encoding Gly17-Gln208 is expressed with a 6His tag at the C-terminus.
Purity:
> 95 % as determined by reducing SDS-PAGE.
Mol Mass:
22.6 kDa
AP Mol Mass:
40 kDa
Formulation:
Lyophilized from a 0.2 µm filtered solution of 20mM PB; 150mM NaCl; pH 7.2.
Shipping:
This product is provided as lyophilized powder which is shipped with ice packs.
Stability and Storage:
Lyophilized proteins are stable for up to 12 months when stored at -20 to -80°C. Reconstituted protein solution can be stored at 4-8°C for 2-7 days. Aliquots of reconstituted samples are stable at < -20°C for 3 months.
