Signal Regulatory Protein ? (SIRP?) is a monomeric approximately 90 kD type I transmembrane glycoprotein. The 504 amino acid human SIRP? contains two Ig-like C1-type domains and one Ig-like V-type domain. SIRP? can express in various tissues, mainly on brain and myeloid cells, including macrophages, neutrophils, dendritic and Langerhans cells. It also can detect in neurons, smooth muscle and endothelial cells. SIRPA is an immunoglobulin-like cell surface receptor for CD47. SIRP? acts as docking protein and induces translocation of PTPN6, PTPN11 and other binding partners from the cytosol to the plasma membrane. SIRP? shows adhesion of cerebellar neurons, neurite outgrowth and glial cell attachment. SIRP? engagement generally produces a negative regulatory signal; it may mediate negative regulation of phagocytosis, mast cell activation and dendritic cell activation
Product Name:
Human CD172a/SIRPA Recombinant Protein (RPES4894)
Product Code:
RPES4894
Size:
10µg
Species:
Human
Expressed Host:
Human Cells
Synonyms:
Tyrosine-Protein Phosphatase Non-Receptor Type Substrate 1, SHP Substrate 1, SHPS-1, Brain Ig-Like Molecule with Tyrosine-Based Activation Motifs, Bit, CD172 Antigen-Like Family Member A, Inhibitory Feceptor SHPS-1, Macrophage Fusion Receptor, MyD-1 Antigen, Signal-Regulatory Protein Alpha-1, Sirp-Alpha-1, Signal-Regulatory Protein Alpha-2, Sirp-Alpha-2, Signal-Regulatory Protein Alpha-3, Sirp-Alpha-3, p84, CD172a, SIRPA, BIT, MFR, MYD1, PTPNS1, SHPS1, SIRP
Accession:
CAA71403.1
Sequence:
Glu31-Arg370
Fusion tag:
C-His
Activity:
Immobilized Human CD47-Fc(Cat: PKSH032703) at 2µg/ml(100 ?l/well) can bind Human SIRPA-His. The ED50 of Human SIRPA-His is 0.49ug/ml .
Endotoxin:
<1.0 EU per µg as determined by the LAL method.
Protein Construction:
Recombinant Human CD172a is produced by our Mammalian expression system and the target gene encoding Glu31-Arg370 is expressed with a 6His tag at the C-terminus.
Purity:
> 95% as determined by reducing SDS-PAGE.
Mol Mass:
38.31 kDa
AP Mol Mass:
45-65 kDa
Formulation:
Lyophilized from a 0.2 µm filtered solution of 20mM PB, 150mM NaCl, pH 7.2.
Shipping:
This product is provided as lyophilized powder which is shipped with ice packs.
Stability and Storage:
Lyophilized proteins are stable for up to 12 months when stored at -20 to -80°C. Reconstituted protein solution can be stored at 4-8°C for 2-7 days. Aliquots of reconstituted samples are stable at < -20°C for 3 months.
