T-Cell Surface Glycoprotein CD3 ? Chain (CD3?) is a single-pass type I membrane protein. CD3? contains 1 Ig-like (immunoglobulin-like) domain and 1 ITAM domain. CD3? is a polypeptide encoded by the CD3E gene on chromosome 11 in humans. The T cell receptor-CD3 complex (TCR/CD3 complex) is involved in T-cell development and several intracellular signal-transduction pathways. This complex is critical for T-cell development and function, and represents one of the most complex transmembrane receptors. The T cell receptor-CD3 complex is unique in having ten cytoplasmic immunoreceptor tyrosine-based activation motifs (ITAMs). TCR/CD3 complex plays an important role in coupling antigen recognition to several intracellular signal-transduction pathways.
Product Name:
Human CD3 Epsilon/CD3E Recombinant Protein (RPES3250)
Immobilized Human CD3E-His at 10 µg/ml(100 ?l/well) can bind Human Anti-CD3. The ED50 of Human CD3E-His is 3.9 ng/ml.
Endotoxin:
<1.0 EU per µg as determined by the LAL method.
Protein Construction:
Recombinant Human T-Cell Surface Glycoprotein CD3 epsilon Chain is produced by our Mammalian expression system and the target gene encoding Asp23-Asp126 is expressed with a 6His tag at the C-terminus.
Purity:
> 90 % as determined by reducing SDS-PAGE.
Mol Mass:
12.8 kDa
AP Mol Mass:
18 kDa
Formulation:
Lyophilized from a 0.2 µm filtered solution of 20mM PB, 150mM NaCl, pH 7.2.
Shipping:
This product is provided as lyophilized powder which is shipped with ice packs.
Stability and Storage:
Lyophilized proteins are stable for up to 12 months when stored at -20 to -80°C. Reconstituted protein solution can be stored at 4-8°C for 2-7 days. Aliquots of reconstituted samples are stable at < -20°C for 3 months.
