Processing of the N-terminal initiator methionine or formylated methionine is an essential cellular process conserved from prokaryotes to eukaryotes. The proteolytic removal of N-terminal methionine from nascent peptides is catalyzed by a family of enzymes known as methionine aminopeptidases (MetAPs) and is essential for cell growth. METAP1 and METAP2 have different substrate specificity due to the differences in both size and shape of the active sites. As a member of the M24 family of metalloproteases, METAP1 plays an important role in G(2)/M phase regulation of the cell cycle and may serve as a promising target for the discovery and development of new anticancer agents.
Product Name:
Human CD32b/FCGR2B Recombinant Protein (RPES4943)
Product Code:
RPES4943
Size:
50µg
Species:
Human
Expressed Host:
HEK293 Cells
Synonyms:
Low Affinity Immunoglobulin Gamma Fc Region Receptor II-b, IgG Fc Receptor II-b, CDw32, Fc-Gamma RII-b, Fc-Gamma-RIIb, FcRII-b, CD32, FCGR2B, FCG2, IGFR2
Accession:
NP_001002274.1
Sequence:
Ala 46-Pro 217
Fusion tag:
C-His
Activity:
Measured by its binding ability in a functional ELISA. Immobilized recombinant human CD32b at 10 µg/ml (100 ?l/well) can bind human IgG2 with a linear range of 0.16-6.4 µg/ml.
Endotoxin:
<1.0 EU per µg of the protein as determined by the LAL method.
Protein Construction:
A DNA sequence encoding the extracellular domain (Ala 46-Pro 217) of mature human CD32b (NP_001002274.1) protein was expressed with a N-terminal signal peptide and a C-terminal polyhistidine tag.
Purity:
> 95 % as determined by reducing SDS-PAGE.
Mol Mass:
20.8 kDa
AP Mol Mass:
25-30 kDa
Formulation:
Lyophilized from sterile PBS, pH 7.4
Shipping:
This product is provided as lyophilized powder which is shipped with ice packs.
Stability and Storage:
Lyophilized proteins are stable for up to 12 months when stored at -20 to -80°C. Reconstituted protein solution can be stored at 4-8°C for 2-7 days. Aliquots of reconstituted samples are stable at < -20°C for 3 months.
