Description
| Product Name: | CD3D & CD3E Heterodimer Recombinant Protein (RPES2817) |
| Product Code: | RPES2817 |
| Size: | 20µg |
| Species: | Human |
| Expressed Host: | HEK293 Cells |
| Synonyms: | T-Cell Surface Glycoprotein CD3 Delta Chain, T-Cell Receptor T3 Delta Chain, CD3d, CD3D, T3D |
| Accession: | P04234&P07766 |
| Sequence: | Met 1-Ala105&Met 1-Asp126 |
| Fusion tag: | |
| Activity: | Measured by its binding ability in a functional ELISA.Immobilized human CD3D & CD3E Heterodimer Protein at 10 µg/ml (100 ?l/well) can bind OKT3-mIgG2a. The EC50 of OKT3-mIgG2a is 0.07-0.17 µg/ml. |
| Endotoxin: | <1.0 EU per µg of the protein as determined by the LAL method. |
| Protein Construction: | A DNA sequence encoding the extracellular domain (Met 1-Ala 105) of human CD3D (P04234) was fused with the C-terminal flag-tagged Fc region of human IgG1 at the C-terminus; constructed the plasmid 1; A DNA sequence encoding the extracellular domain (Met 1-Asp 126) of human CD3E (P07766) was fused with the C-terminal His-tagged Fc region of human IgG1 at the C-terminus; constructed the plasmid 2. The two plasmids were co-expressed and the human CD3D/CD3E heterodimer was purified. |
| Purity: | > 90 % as determined by reducing SDS-PAGE. |
| Mol Mass: | 77.5 (37.5+40? kDa |
| AP Mol Mass: | 45-50 kDa |
| Formulation: | Lyophilized from sterile PBS; pH 7.4 |
| Shipping: | This product is provided as lyophilized powder which is shipped with ice packs. |
| Stability and Storage: | Lyophilized proteins are stable for up to 12 months when stored at -20 to -80°C. Reconstituted protein solution can be stored at 4-8°C for 2-7 days. Aliquots of reconstituted samples are stable at < -20°C for 3 months. |
