CD47 contains 1 Ig-like V-type (immunoglobulin-like) domain and is a receptor for the C-terminal cell-binding domain of thrombospondin. CD47 may help to regulate membrane transport and signal transmission. CD47 is involved in the elevation of intracellular calcium concentration that occurs when cells bind to the extracellular matrix. It is widely distributed on normal adult tissues; it is particularly common in some epithelias and the brain. CD47 may play a role in membrane transport and/or integrin dependent signal transduction. CD47 may prevent the premature elimination of red blood cells. It may be linked to cell membrane permeability changes that result from viral infection. CD47 serves as a cell adhesion receptor for THBS1 on platelets, and it also plays a role in the regulation of integrins. It also plays an important role in memory formation and synaptic plasticity in the hippocampus. Human CD47 Recombinant Protein is a highly pure recombinant protein developed by Assay Genie for use in a range of applications.
Product Name:
Human CD47 Recombinant Protein (RPES0500)
Product Code:
RPES0500
Size:
50µg
Species:
Human
Expressed Host:
HEK293 Cells
Synonyms:
IAP,MER6,OA3
Accession:
NP_942088.1
Sequence:
Met 1-Pro 139
Fusion tag:
Activity:
Measured by its ability to bind human SIRPA-His in functional ELISA. Measured by its ability to bind mouse SIRPA-His(Cat: PKSM040419?in functional ELISA.
Endotoxin:
<1.0 EU per µg as determined by the LAL method.
Protein Construction:
A DNA sequence encoding the human CD47 (NP_942088.1) (Met1-Pro139) was expressed with six amino acids (LEVLFQ) at the C-terminus.
Purity:
> 90 % as determined by reducing SDS-PAGE.
Mol Mass:
14.5 kDa
Formulation:
Lyophilized from sterile PBS; pH 7.4
Shipping:
This product is provided as lyophilized powder which is shipped with ice packs.
Stability and Storage:
Lyophilized proteins are stable for up to 12 months when stored at -20 to -80°C. Reconstituted protein solution can be stored at 4-8°C for 2-7 days. Aliquots of reconstituted samples are stable at < -20°C for 3 months.
