Ciliary Neurotrophic Factor (CNTF) is a potent survival factor for neurons and oligodendrocytes. This protein has also been discovered to prevent motor nerve regeneration after damage. CNTF is highly conserved across species, with cross-species effects. CNTF proteins from both humans and rats have 83% homology in their amino acid sequence. CNTF is structurally related to IL6, IL11, LIF and OSM. In their receptor complexes, all of these four-helix bundle cytokines include gp130 as a signal-transducing subunit. Due to their lack of a signal sequence, CNTFs such as FGF acidic, basic, and PD-ECGF (platelet-derived endothelial cell growth factor) are unable to be secreted via traditional secretion pathways. The mechanism underlying the release of CNTF is unknown. Human CNTF Recombinant Protein is a highly pure recombinant protein developed by Assay Genie for use in a range of applications.
Product Name:
Human CNTF Recombinant Protein (RPES3472)
Product Code:
RPES3472
Size:
10µg
Species:
Human
Expressed Host:
E.coli
Synonyms:
Ciliary Neurotrophic Factor, CNTF
Accession:
P26441
Sequence:
Ala2-Met200
Activity:
Measured in a cell proliferation assay using TF?1 human erythroleukemic cells. The ED50 for this effect is 25 ng/ml.
Endotoxin:
<1.0 EU per µg as determined by the LAL method.
Protein Construction:
Recombinant Human Ciliary Neurotrophic Factor is produced by our E.coli expression system and the target gene encoding Ala2-Met200 is expressed.
Purity:
> 95% as determined by reducing SDS-PAGE.
Mol Mass:
22.93 kDa
AP Mol Mass:
25 kDa
Formulation:
Lyophilized from a 0.2 µm filtered solution of PBS,1mM Cysteine,PH7.4.
Shipping:
This product is provided as lyophilized powder which is shipped with ice packs.
Stability and Storage:
Lyophilized proteins are stable for up to 12 months when stored at -20 to -80°C. Reconstituted protein solution can be stored at 4-8°C for 2-7 days. Aliquots of reconstituted samples are stable at < -20°C for 3 months.
