Ubiquinone biosynthesis protein COQ7 homolog; also known as Coenzyme Q biosynthesis protein 7 homolog; Timing protein clk-1 homolog and COQ7; is a mitochondrion inner membrane and peripheral membrane protein which belongs to the COQ7 family. It is expressed dominantly in heart and skeletal muscle. COQ7 is synthesized as a preprotein that is imported into the mitochondrial matrix; where the sequence is cleaved off and the mature protein becomes loosely associated with the inner membrane. This enzyme is responsible for the hydroxylation of 5-demethoxyubiquinone to 5-hydroxyubiquinone. Human COQ7 protein is mostly helical; and contains an alpha-helical membrane insertion. It has a potential N-glycosylation site; a phosphorylation site for protein kinase C and another for casein kinase II; and three N-myristoylation sites. COQ7 is involved in lifespan determination in ubiquinone-independent manner. It is also involved in ubiquinone biosynthesis. COQ7 is potential central metabolic regulator.
<1.0 EU per µg of the protein as determined by the LAL method.
Protein Construction:
A DNA sequence encoding the human COL9A1 (P20849-3) (Met1-Pro328) was fused with Fc region of mouse IgG at the C-terminus.
Purity:
> 95 % as determined by reducing SDS-PAGE.
Mol Mass:
59.2 kDa
AP Mol Mass:
62-72 kDa
Formulation:
Lyophilized from sterile PBS; pH 7.4
Shipping:
This product is provided as lyophilized powder which is shipped with ice packs.
Stability and Storage:
Lyophilized proteins are stable for up to 12 months when stored at -20 to -80°C. Reconstituted protein solution can be stored at 4-8°C for 2-7 days. Aliquots of reconstituted samples are stable at < -20°C for 3 months.
