5a is a glycoprotein that is generated enzymatically and belongs to the anaphylatoxins family of structurally and functionally related proteins. C5a convertase, an enzyme that transforms the C5 alpha-chain into C5a, creates the C5a fragment. Human C5a has four alpha-helices plus three intrachain disulfide bonds that create a triple loop structure. Human C5a is 60% and 54% identical to mouse and rat C5a, respectively. C5a binds to a signaling G-protein coupled receptor (C5aR/CD88) as well as a nonsignaling GPCR called C5L2. Cd88 activation induces neutrophil chemotaxis and endothelial cell activation. It also causes macrophages and neutrophils to release hydrogen peroxide, as well as stimulating basophiles and mast cells to secrete histamine. Human Complement C5a Recombinant Protein is a highly pure recombinant protein developed by Assay Genie for use in a range of applications.
Product Name:
Human Complement C5a Recombinant Protein (RPES4028)
Recombinant Human C5a is produced by our E.coli expression system and the target gene encoding Thr678-Arg751 is expressed.
Purity:
> 95% as determined by reducing SDS-PAGE.
Mol Mass:
8.4 kDa
AP Mol Mass:
12 kDa
Formulation:
Lyophilized from a 0.2 µm filtered solution of PBS, pH 7.4.
Shipping:
This product is provided as lyophilized powder which is shipped with ice packs.
Stability and Storage:
Lyophilized proteins are stable for up to 12 months when stored at -20 to -80°C. Reconstituted protein solution can be stored at 4-8°C for 2-7 days. Aliquots of reconstituted samples are stable at < -20°C for 3 months.
