The complement system is an important component of natural immunity and the first line of defense in the battle against invading organisms. Complement 3 (C3) is the most common component of the complement cascade and the convergent point for all three major complement activation pathways: classical, alternative, and mannose-binding lectin pathways. Complement activation leads to the formation of C3 convertase, which cleaves C3 into the key effector molecules, C3a (anaphylatoxin) and C3b (opsonin), which then drive microbe removal. C3a activates the C3aR (C3ar) by binding to it, causing profound anaphylatoxin activity, including enhanced vascular permeability and mast cell degranulation, inflammation, and leukocyte activation. Complement Component C3a/C3a Recombinant Protein is a highly pure recombinant protein developed by Assay Genie for use in a range of applications.
Complement Component C3a/C3a Recombinant Protein (RPES0860)
Complement Component C3a, C3a, Anaphylatoxin
<1.0 EU per µg as determined by the LAL method.
Recombinant Human Complement Component C3a is produced by our E.coli expression system and the target gene encoding Ser672-Arg748 is expressed.
> 95 % as determined by reducing SDS-PAGE.
AP Mol Mass:
Lyophilized from a 0.2 µm filtered solution of 20mM PB, 150mM NaCl, pH7.4.
This product is provided as lyophilized powder which is shipped with ice packs.
Stability and Storage:
Lyophilized proteins are stable for up to 12 months when stored at -20 to -80°C. Reconstituted protein solution can be stored at 4-8°C for 2-7 days. Aliquots of reconstituted samples are stable at < -20°C for 3 months.