Recombinant Human Creatine Kinase BB/CKB Protein (His Tag) (RPES3312)

SKU:
RPES3312
€230

Description

ELISA Kit Technical Manual

Recombinant Human Creatine Kinase BB/CKB Protein (His Tag)

Background:Creatine kinase B-type (CKB) belongs to the ATP:guanido phosphotransferase family. It has dimer of identical or non-identical chains with MM being the major form in skeletal muscle and myocardium. MB exists in myocardium, and BB exists in many tissues, especially brain. CKB reversibly catalyzes the transfer of phosphate between ATP and various phosphogens (e.g. creatine phosphate). Creatine kinase isoenzymes play a central role in energy transduction in tissues with large, fluctuating energy demands, such as skeletal muscle, heart, brain and spermatozoa. Clinically, creatine kinase is assayed in blood tests as a marker of myocardial infarction (heart attack), rhabdomyolysis (severe muscle breakdown), muscular dystrophy, autoimmune myositides and acute renal failure.
Name:Recombinant Human Creatine Kinase BB/CKB Protein (His Tag)
Size:10µg
Assay Genie SKU:RPES3312
Synonyms:B-CK; CKB; Creatine Kinase BB
Species:Human
Expression host:E.coli
Sequence:Met1-Lys381
Accession:P12277
Mol Mass:44.8 kDa
AP Mol Mass:50 kDa
Tag:N-His
Purity:> 90% as determined by reducing SDS-PAGE.
Endotoxin:< 1.0 EU per ?g as determined by the LAL method.
Storage:Store at < -20°C, stable for 6 months. Please minimize freeze-thaw cycles.
Shipping:This product is provided as liquid. It is shipped at frozen temperature with blue ice.Upon receipt, store it immediately at<-20°C.
Formulation:Supplied as a 0.2 um filtered solution of 20mM TrisHCl,150mM NaCl,10% Glycerol,pH 7.5.
Reconstitution:Please refer to the printed manual for detailed information.
UniProt Protein Function:Function: Reversibly catalyzes the transfer of phosphate between ATP and various phosphogens (e.g. creatine phosphate). Creatine kinase isoenzymes play a central role in energy transduction in tissues with large, fluctuating energy demands, such as skeletal muscle, heart, brain and spermatozoa.
UniProt Protein Details:

Catalytic activity: ATP + creatine = ADP + phosphocreatine.

Subunit structure: Dimer of identical or non-identical chains. With MM being the major form in skeletal muscle and myocardium, MB existing in myocardium, and BB existing in many tissues, especially brain.

Subcellular location: Cytoplasm.

Sequence similarities: Belongs to the ATP:guanido phosphotransferase family.Contains 1 phosphagen kinase C-terminal domain.Contains 1 phosphagen kinase N-terminal domain.

NCBI Summary:The protein encoded by this gene is a cytoplasmic enzyme involved in energy homeostasis. The encoded protein reversibly catalyzes the transfer of phosphate between ATP and various phosphogens such as creatine phosphate. It acts as a homodimer in brain as well as in other tissues, and as a heterodimer with a similar muscle isozyme in heart. The encoded protein is a member of the ATP:guanido phosphotransferase protein family. A pseudogene of this gene has been characterized. [provided by RefSeq]
UniProt Code:P12277
NCBI GenInfo Identifier:125294
NCBI Gene ID:1152
NCBI Accession:P12277.1
UniProt Secondary Accession:P12277,Q2LE07, Q6FG40, Q9UC66, A8K236, B2R5R4,
UniProt Related Accession:P12277
Molecular Weight:42,644 Da
NCBI Full Name:Creatine kinase B-type
NCBI Synonym Full Names:creatine kinase, brain
NCBI Official Symbol:CKB
NCBI Official Synonym Symbols:B-CK; CKBB
NCBI Protein Information:creatine kinase B-type; creatine kinase-B; creatine kinase B chain
UniProt Protein Name:Creatine kinase B-type
UniProt Synonym Protein Names:B-CK; Creatine kinase B chain
Protein Family:Creatine kinase
UniProt Gene Name:CKB
UniProt Entry Name:KCRB_HUMAN
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Additional Information

Product type:
Recombinant Protein
Host species:
E.coli
Reactivity:
Human
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