Casein kinase I gamma 2 isoform (CSNK1G2); a member of the large casein kinase I (CKI) subfamily; protein kinase superfamily. It may affect the development of brain; and associate with vesicular trafficking and neurotransmitter releasing from small synaptic vesicles. The CKI family includes several other isoforms (alpha; beta; gamma; and delta). Dishevelled (Dsh); another positive component of the Wnt pathway; becomes phosphorylated in response to Wnt signals. All the CKI isoforms; with the exception of gamma; increase the phosphorylation of Dsh in vivo. Casein kinase 1 gamma (CK1gamma; or CSNK1G) is associated with the cell membrane and binds to LRP. CK1gamma was found to be needed for Wnt signaling through Wnt receptor LRP. CSNK1G2 inhibits Smad3-mediated TGF-beta responses including induction of target genes and cell growth arrest; and this inhibition is dependent on CSNK1G2 kinase activity. The overexpression of CSNK1G2 in human cancers; may act as an oncoprotein during tumorigenesis. In addition; as an MTA1s-binding protein; CSNK1G2 could further potentiate the estrogen receptor (ER) corepressive function of MTA1s.
Product Name:
Human CSNK1G2 Recombinant Protein (RPES1443)
Product Code:
RPES1443
Size:
10µg
Species:
Human
Expressed Host:
E.coli
Synonyms:
Casein kinase I isoform gamma-2, CK1G2, CSNK1G2
Accession:
P78368-1
Sequence:
Met 18-Lys415
Fusion tag:
N-6His
Endotoxin:
<1.0 EU per µg as determined by the LAL method.
Protein Construction:
Recombinant Human Casein kinase I isoform gamma-2 is produced by our E.coli expression system and the target gene encoding Met18-Lys415 is expressed with a 6His tag at the N-terminus.
Purity:
> 90 % as determined by reducing SDS-PAGE.
Mol Mass:
47.6 kDa
AP Mol Mass:
47.6 kDa
Formulation:
Supplied as a 0.2 µm filtered solution of 20mM Tris;500mM NaCl;10%Glycerol;1mM DTT; pH 8.0.
Shipping:
This product is provided as liquid. It is shipped at frozen temperature with blue ice/gel packs.Upon receipt; store it immediately at<-20°C.
Stability and Storage:
Store at < -20°C, stable for 6 months. Please minimize freeze-thaw cycles.
